Intestinal Differentiated Cells

Methods and Protocols

AvPaloma Ordóñez-Morán

Häftad, Engelska, 2023

Del i serien Methods in Molecular Biology

1 712 kr

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Beskrivning

Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells.

Produktinformation

Utgivningsdatum:2023-06-13
Mått:178 x 254 x 16 mm
Vikt:547 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:275
Förlag:Springer-Verlag New York Inc.
ISBN:9781071630785

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Differentiated epithelial cells of the gut.- Identification of differentiated intestinal epithelial cells using immunostaining and fluorescence microscopy.- Novel approach to measure Trans Epithelial Electrical Resistance in intestinal cells.- In vivo model for isolating epithelial cells of the anorectal transition zone.- In vitro and in vivo assays for testing retinoids effect on intestinal progenitors’ lineage commitments.- TORNADO-seq: a protocol for high-throughput targeted RNA-seq based drug screening in organoids.- Epigenetic and transcriptional dynamics of Notch program in intestinal differentiation.- Defining anorectal transition zone heterogeneity using single-Cell RNA sequencing.- Directed differentiation of murine and human small intestinal organoids towards all mature lineages.- Modeling Notch activity and lineage decisions using intestinal organoids.- Generation of fetal Intestinal organoids and their maturation into adultintestinal cells.- Visualization of differentiated cells in 3D- and 2D-intestinal organoid cultures.- In-vitro culture and histological evaluation of 3D organotypic cultures.- Fluorescence intensity and fluorescence lifetime imaging (FLIM) microscopies of cell differentiation in the small intestinal organoids using cholera toxin.- In vitro morphogenesis and differentiation of human intestinal epithelium in a gut-on-a-chip.- Co-culturing human intestinal enteroid monolayers with innate immune cells.- Measuring cell differentiation in colorectal cancer.- Intestinal cell differentiation and phenotype in 2D and 3D cell culture models.- Confocal laser scanning imaging of cell junctions in human colon cancer cells.- Automated quantitative analysis of shape features in human epithelial monolayers and spheroids generated from colorectal cancer cells.