Tissue-Resident Macrophages

Methods and Protocols

AvElvira Mass

Inbunden, Engelska, 2023

Del i serien Methods in Molecular Biology

3 504 kr

Beställningsvara. Skickas inom 5-8 vardagar. Fri frakt över 249 kr.

Beskrivning

The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease.

Produktinformation

Utgivningsdatum:2023-08-29
Mått:178 x 254 x 38 mm
Vikt:1 303 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:576
Förlag:Springer-Verlag New York Inc.
ISBN:9781071634363

Utforska kategorier

Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Macrophage Development and Function.- Fate Mapping Macrophages: From Ontogeny to Functions.- Studying Autophagy in Microglia: Overcoming the Obstacles.- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq.- Isolation of Tissue Macrophages in Adult Zebrafish.- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ.- Phenotyping of Macrophages in Human Immune System Mice.- Fate-Mapping of Yolk Sac-Derived Macrophages.- Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages.- Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue.- Isolation and Flow Cytometry Analysis of Macrophages from the Dermis.- Isolation and Flow Cytometry Analysis of Macrophages from the Kidney.- Isolation and Flow Cytometry Analysis of Intestinal Macrophages.- Isolation and Characterization of Testis Macrophages Using Flow Cytometry.- Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy.- Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages.- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach.- Spectral Flow Cytometry Analysis of Resident Tissue Macrophages.- Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging.- 3D Imaging of Macrophages in Complete Organs.- Whole-Mount Imaging of Adipose Tissue Macrophages.- Functional In Vivo Imaging of Macrophages.- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy.- Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection.- Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH.- Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA).- Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo.- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparationand Inflammasome Stimulation.- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.- Detection of G-Quadruplex DNA Structures in Macrophages.- Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models.- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis.- Automated Cell Counting of Macrophages In Situ.- Morphometric Analyses of Macrophages.- Combined Analysis of mRNA Expression and Open Chromatin in Microglia.