Bacterial Cell Wall

Methods and Protocols

AvHung Ton-That

Häftad, Engelska, 2024

Del 2727 i serien Methods in Molecular Biology

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Beskrivning

This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens.

Produktinformation

Utgivningsdatum:2024-10-12
Mått:178 x 254 x 14 mm
Vikt:482 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:240
Förlag:Springer-Verlag New York Inc.
ISBN:9781071634936

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall.- Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography.- Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy.- Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi.- Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis.- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum.- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids.- Type I Lipoteichoic Acid (LTA) Detection by Western Blot.- Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy.- Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris.- Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography.- Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy.- Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase.- Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins.- Tracking Cell Wall Anchored Proteins in Gram-Positive Bacteria.- Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy.- Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers.- Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy.