Cilia

Methods and Protocols

AvVito Mennella

Inbunden, Engelska, 2023

Del i serien Methods in Molecular Biology

2 115 kr

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Beskrivning

Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells;

Produktinformation

Utgivningsdatum:2023-10-20
Mått:178 x 254 x 22 mm
Vikt:751 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:278
Förlag:Springer-Verlag New York Inc.
ISBN:9781071635063

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium.- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures.- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.- Expansion Microscopy of Ciliary Proteins.- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes.- Structural Analysis of Sperm Centrioles Using N-STORM.- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets.- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.- Proximity Mapping of Ciliary Proteins by BioID.- Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags.- Primary Human Nasal Epithelial Cell Culture.- BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation.- High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models.- Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods.- Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.