Tissue Morphogenesis

Methods and Protocols

AvCeleste M. Nelson

Inbunden, Engelska, 2024

Del 2805 i serien Methods in Molecular Biology

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Beskrivning

This second edition guides readers through experimental and computational techniques on the study of tissue morphogenesis, with a specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis.

Produktinformation

Utgivningsdatum:2024-07-16
Mått:178 x 254 x 19 mm
Vikt:663 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:230
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071638538

Utforska kategorier

Reproduktionsmedicin inom Medicin
Biokemisk teknik inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin

Innehållsförteckning

Isolation, culture, and phenotypic analysis of murine lung organoids.- Using 3-dimensional cultures to propagate genetically modified lung organoids.- High-throughput assembly of compositionally controlled 3D cell communities for developmental engineering.- Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors.- Engineered heart tissues for standard 96-well tissue culture plates.- Building biomaterials to mimic 3D cell-cell junctions.- Bioprinting cell-laden hydrogels for studies of epithelial tissue morphogenesis.- Genome-wide profiling of cis-regulatory elements in mammalian skin.- Multi-labeling live imaging to quantitate gene expression dynamics during Drosophila embryonic development.- PyJAMAS: Python-based analysis of biological images.- Mechanical compression of Drosophila embryos using rapid fabrication microfluidic devices.- Lumen pressure modulation in chicken embryos.- Quantifying spatial patterns of tissue stiffness within the embryonic mouse kidney.- Evaluating planar cell polarity in the developing mouse epidermis.- Purification of planarian stem cells using a Draq5-based FACS approach.- Arabidopsis root microbiome microfluidic (ARMM) device for imaging bacterial colonization and morphogenesis of Arabidopsis roots.