Hepatitis B Virus

Methods and Protocols

AvHaitao Guo,Andrea Cuconati

Inbunden, Engelska, 2024

Del 2837 i serien Methods in Molecular Biology

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Beskrivning

This detailed new edition collects state-of-the-art methods in the fields of hepatitis B virus (HBV) molecular biology and immunology.

Produktinformation

Utgivningsdatum:2024-07-24
Mått:178 x 254 x 21 mm
Vikt:736 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:272
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071640265

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Stem Cell-Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection.- Primary Duck Hepatocyte Culture and Duck Hepatitis B Virus Infection Model.- Hepatitis B Virus Covalently Closed Circular DNA Chromatin Immunoprecipitation Assay.- Analysis of HBV cccDNA Minichromosome Accessibility by MNase-qPCR and High Throughput Sequencing.- Circular Chromosome Conformation Capture (4C-Seq) Analysis of HBV-Host Chromosome DNA Interactome.- Analysis of N6-Methyladenosine Modification of HBV RNA by Methylated RNA Immunoprecipitation.- Structural Analysis of the Hepatitis B Virus RNA Encapsidation Signal ɛ by Selective 2′-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE).- A Particle Gel Assay for Detection of Intracellular Hepatitis B Virus Subviral Particles in Cultured Cells.- Detection of Hepatitis B Virus Covalently Closed Circular DNA and Intermediates in Its Formation.- Rapid and Reliable Protein-Free HBV DNA Extraction and Sensitive Branched DNA Southern Blot Assay.- A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture.- A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue.- Detection and Quantification of HBV Transcripts by Full-Length 5'RACE-PCR.- One-Step Reverse Transcriptase qPCR Method for Serum Hepatitis B Virus RNA Quantification.- Method for Quantitative HDV RNA Detection: I, Manual Workflow (Serum and Liver Tissue) and II, Fully Automated High Throughput Workflow for Diagnostic Use.- The Cre/loxP-Based Recombinant HBV cccDNA System In Vitro and In Vivo.- Modeling HBV Infection and Therapy in Immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (FAH) Knockout Mice with Human Chimeric Liver.- Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication.- Peptide-Loaded HLA Class I Tetramer-Associated Magnetic Bead-Based Enrichment of HBV-Specific CD8+ T Cells.- Optimized Ex Vivo Fluorospot Assay to Measure Multi-Functional HBV-Specific T Cell Frequencies in Chronic Hepatitis B Patients.- Ex Vivo Fluorescent Labeling of HBV-Specific B Cells in Chronic Hepatitis B Patients.- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV Ribonuclease H Inhibitors.