Bacterial Extracellular Vesicles

Methods and Protocols

AvSteven Jay,Nicholas Pirolli

Inbunden, Engelska, 2024

Del 2843 i serien Methods in Molecular Biology

2 463 kr

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Beskrivning

Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function In Vitro, preparation and functionalization of therapeutic Bacterial Extracellular Vesicles, and delivery and tracking of Bacterial Extracellular Vesicles In Vivo.

Produktinformation

Utgivningsdatum:2024-08-15
Mått:178 x 254 x 21 mm
Vikt:736 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:274
Upplaga:2024
Förlag:Springer-Verlag New York Inc.
ISBN:9781071640548

Utforska kategorier

Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Methods to Analyze the Surface Composition of Bacterial Membrane Vesicles.- Analysis of Bacterial Extracellular Vesicles by Immunogold Transmission Electron Microscopy.- Methods for the Characterization of the Colloidal Properties of Bacterial Membrane Vesicles.- Methods for Bioinformatic Prediction of Genuine sRNAs from Outer Membrane Vesicles.- Screening for Genes Involved in Outer Membrane Vesicle Biogenesis in Bacteroides thetaiotaomicron.- Use of Synchrotron Radiation Circular Dichroism to Analyze the Interaction and Insertion of Proteins into Bacterial Outer Membrane Vesicles.- Methods for Assessing Plant Immune System Activation by Bacterial Extracellular Vesicles and Other Elicitors.- Methods for Studying Fusion of Bacterial Extracellular Vesicles with Intact Bacteria and Host Cells.- Multiple Particle Tracking: A Method for Probing Biologically Relevant Mobility of Bacterial Extracellular Vesicles.- Separation of Bacterial Extracellular Vesicles Via High Performance Anion Exchange Chromatography.- Spray Drying of Bacterial Membrane Vesicles for Vaccine Delivery.- Engineering Outer Membrane Vesicles to Carry Enzymes: Encapsulation, Isolation, Characterization, and Modification.- On-Demand Vaccine Production Via Dock-and-Display of Biotinylated Antigens on Bacterial Extracellular Vesicles.- Dose Determination and Administration of Bacterial Extracellular Vesicles for In Vivo Preclinical Studies.- Assessing In Vivo Bacterial Extracellular Vesicle Biodistribution Using Fluorescent Lipophilic Membrane Stains.- Molecular approaches to measure bacterial extracellular vesicles in tissue samples.