Ocular Regeneration

Methods and Protocols

AvJeffrey M. Gross

Inbunden, Engelska, 2024

Del 2848 i serien Methods in Molecular Biology

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Beskrivning

This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology.

Produktinformation

Utgivningsdatum:2024-09-07
Mått:178 x 254 x 23 mm
Vikt:787 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:300
Upplaga:2025
Förlag:Springer-Verlag New York Inc.
ISBN:9781071640869

Utforska kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin
Oftalmologi inom Medicin

Innehållsförteckning

Isolation, Culture, and Quality Assessment of Clinical-Grade Corneal Stromal Stem Cells.- Generation of Functional Lentoid Bodies from Human Induced Pluripotent Stem Cells.- Lens Regeneration: The Application of iSyTE and In Silico Approaches to Evaluate Gene Expression in Lens Organoids.- Trabecular Meshwork Regeneration for Glaucoma Treatment Using Stem Cell-Derived Trophic Factors.- Models of Photoreceptor Degeneration in Adult Zebrafish.- Isolation and Preparation of Embryonic Zebrafish Retinal Cells for Single-Cell RNA Sequencing.- Nuclei Isolation from Ocular Tissues of the Embryonic Chicken for Single-Nucleus Profiling.- Experimental Framework for Assessing Mouse Retinal Regeneration through Single Cell RNA-Sequencing.- Isolation and Characterization of Extracellular Vesicles to Activate Retina Regeneration.- In Vivo Imaging of Rodent Retina in Retinal Disease.- Retinal Explant Culture from Mouse, Human, and Non-Human Primates and Its Applications in Vision Research.- Generating ESC-Derived RGCs for Cell Replacement Therapy.- Generation of Induced-Primary Retinal Pigment Epithelium from Human Retinal Organoids.- Automated Phenotypic Screening Platform for Identifying Factors that Affect Cell Regeneration Kinetics In Vivo.- Chromatographic Purification and Polishing of AAV Particles.- Controlled Release of Molecules to Enhance Cell Survival and Regeneration.- Development and Validation of a Proximity Labeling Fusion Protein Construct to Identify the Protein-Protein Interactions of Transcription Factors.