This updated volume provides a concise set of protocols on the conceptual principles and practical considerations pertinent to research in mitochondrial function. Driven by the impact of drug- and environmental chemical-induced disturbances on mitochondrial function, hereditary deficiencies, and the age-related decline in bioenergetic performance, this book explores the latest approaches developed in this field by presenting practical principles and assays for quantitative assessments of various parameters reflecting aspects of mitochondrial bioenergetics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mitochondrial Bioenergetics: Methods and Protocols, Third Edition serves as an ideal guide for scientists endeavoring to enhance the quality of mitochondrial research.