Mitochondrial Bioenergetics

Methods and Protocols

AvCarlos M. Palmeira,Paulo J. Oliveira

Inbunden, Engelska, 2024

Del 2878 i serien Methods in Molecular Biology

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Beskrivning

This updated volume provides a concise set of protocols on the conceptual principles and practical considerations pertinent to research in mitochondrial function.

Produktinformation

Utgivningsdatum:2024-11-16
Mått:178 x 254 x 23 mm
Vikt:785 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:295
Upplaga:3
Förlag:Springer-Verlag New York Inc.
ISBN:9781071642634

Utforska kategorier

Innehållsförteckning

Evaluation of Respiration with a Clark-Type Electrode in Isolated Mitochondria, Intact and Permeabilized Cells, and Explants from Animal Tissues.- High-Resolution Respirometry Methodology for Bioenergetic and Metabolic Studies in Intact Brain Slices.- Plate-Based Measurement of Respiration by Isolated Mitochondria and by Intact Cells.- Metabolic Phenotyping of Synaptic Mitochondria Using MitoPlatesTM and Synaptoneurosomes.- Characterization of the Mitochondria Function and Metabolism in Skin Fibroblasts Using the Biolog MitoPlate S-1.- Measurement of Mitochondrial ROS Formation.- Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria.- The Relation between Mitochondrial Membrane Potential and Reactive Oxygen Species Formation.- Creating Physiological Cell Environments In Vitro: Adjusting Cell Culture Media Composition and Oxygen Levels to Investigate Mitochondrial Function and Cancer Metabolism.- Imaging Mitochondrial Axonal Transport in Human Induced Pluripotent Stem Cell-Derived Neurons.- Understanding Mitochondrial and Lysosomal Dynamics by Fluorescent Microscopy.- Mitochondrial Morphofunctional Profiling in Primary Human Skin Fibroblasts Using TMRM and Mitotracker Green Co-Staining.- Absolute Quantification of Cellular and Cell-Free Mitochondrial DNA Copy Number from Human Blood and Urinary Samples Using Real Time Quantitative PCR.- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells.- Methodologies for Mitochondrial Omic Profiling during Spaceflight.