Mitochondrial Bioenergetics

Methods and Protocols

AvCarlos M. Palmeira,António J. Moreno

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 19 mm
Vikt:655 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:333
Förlag:Humana Press Inc.
ISBN:9781493958375

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.- High-Resolution Respirometry. OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle.- High Throughput Analysis of Mitochondrial Oxygen Consumption. – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria. - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells.- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.-Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria.-Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function.-Measurement of Proton Leak and Electron Leak in Isolated Mitochondria.-Relation between Mitochondrial Membrane Potential and ROS Formation.- Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport .-Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy.- Mitochondrial Permeability Transition Pore and Calcium Handling.- Imaging of Mitochondrial pH Using SNARF-1.- Redox Equivalents and Mitochondrial Bioenergetics.-NMR Methodologies for Studying Mitochondrial Bioenergetics.-Computational Modeling of Mitochondrial Function.