Autophagy in Development and Disease

Methods and Protocols

AvKursad Turksen

Inbunden, Engelska, 2025

Del 2879 i serien Methods in Molecular Biology

2 432 kr

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Beskrivning

This detailed book presents state-of-the-art protocols from scientists actively working in autophagy research, a crucial cellular process that regulates numerous cellular functions.

Produktinformation

Utgivningsdatum:2025-02-18
Mått:178 x 254 x 24 mm
Vikt:821 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:316
Förlag:Springer-Verlag New York Inc.
ISBN:9781071642672

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Autophagy Analysis: A Step-by-Step Simple Practical Guide for Immunofluorescence and Western Blotting.- Monitoring Autophagy During Drosophila Oogenesis.- Assessment of Non-Canonical Functions of the Autophagy Proteins in LC3-Associated Phagocytosis (LAP) and LC3-Associated Endocytosis (LANDO).- Monitoring Autophagy with GFP-LC3 Reporter.- Investigating Protein-Protein Interactions of Autophagy-Involved TNIP1.- Proteomic Methods to Study Autophagy in Skin Exposed to Pollutants.- The Chorioallantoic Membrane (CAM) Assay for the Analysis of Starvation-Induced Autophagy.- Keeping an Eye Out for Autophagy in the Cornea: Sample Preparation for Single Cell RNA Sequencing.- Evaluation of Autophagy in Conjunctival Fibroblasts.- Real-Time Visualization of Cholesterol Trafficking in Human Granulosa Cells Using Confocal Live Cell Microscopy as a Tool to Study the Novel Role of Autophagy in Sex Steroid Synthesis.- Visualizing Lipophagy as a New Mechanism of the Synthesis of Sex Steroids in Human Ovary and Testis Using Immunofluorescence Staining Method.- Characterization of Bitter Taste Receptor Dependent Autophagy in Oral Epithelial Cells.- Involvement of Autophagic Machinery in Neuropathogenesis: Targeting and Relevant Methods of Detection.- Assessment of Autophagy in Leishmania Parasites.- Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining.- Assessing Autophagy Flux in Glioblastoma Temozolomide Resistant Cells.- A Lipidomics Approach to Determine the Role of Lipids and Its Crosstalk with Autophagy in Lung Cancer Metastasis.- Evaluation of Mitochondrial Phagy (Mitophagy) in Human Non-Small Adenocarcinoma Tumor Cells.- Assessment of Stiffness-Dependent Autophagosome Formation and Apoptosis in Embryonal Rhabdomyosarcoma Tumor Cells.- Principles of Hanging Drop Method (Spheroid Formation) in Cell Culture.- Protocol for Testing the Effects of ssDNA Aptamer in HeLa and MCF-7.