Intracellular Lipid Transport

Methods and Protocols

AvGuillaume Drin

Inbunden, Engelska, 2024

Del 2888 i serien Methods in Molecular Biology

2 644 kr

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Beskrivning

This fully updated volume explores different classes of lipid transporters, such as lipid transfer proteins, flippases, and scramblases, and significant advances in solving their biological functions.

Produktinformation

Utgivningsdatum:2024-12-20
Mått:178 x 254 x 24 mm
Vikt:836 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:325
Upplaga:2
Förlag:Springer-Verlag New York Inc.
ISBN:9781071643174

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

Selective Labeling of Phosphatidylcholine to Track Their Translocation Between Organelles in Living Cells.- Measurement of ORP10-Mediated Lipid Countertransport at ER-Endosome Membrane Contact Sites Via a Chemically-Induced Dimerization Strategy.- Analysis of Phosphatidylinositol Transfer at ER-PM Contact Sites in Receptor-Stimulated Live Cells.- Use of the D4H Probe to Track Sterols in Yeast.- Maistero-2, a Novel Probe for Sterols: Application for Visualizing Cellular Cholesterol.- Using GRAM Domain-Based Biosensors to Monitor Accessible Cholesterol Distribution in Live Cells.- Monitoring Accessible Cholesterol Levels in Immune Cells.- Methods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes.- HEK293 Cell-Based Assay to Measure the Lipid Scrambling Activity of TMEM16 Family Members.- Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells.- Advanced Method for the In Vivo Measurements of Lysophospholipid Translocation Across the Inner (Cytoplasmic) Membrane of Escherichia coli.- Measurement of Lipid Transport in Mitochondria by the MTL Complex.- Determining ATG2 Localization During Autophagosome Formation in Mammalian Cells.- Use of Bicelle-Generated Lipid Bilayer Vesicle Nanoparticles for In Vitro Measurement of Lipid Intermembrane Transport.- DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.- Galactocerebroside Lipid Nanotubes, a Model Membrane System for Studying Membrane-Associated Proteins on a Molecular Scale.- In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs.- Determining the Relative Affinity of ORPs for Lipid Ligands Using Fluorescence and Thermal Shift Assays.- Atomistic Simulations and Analysis of Peripheral Membrane Proteins with Model Lipid Bilayers.- In Silico Strategies for Characterizing Inner Cavities of Lipid-Binding Proteins.