RAVIN NARAIN, PhD, is Associate Professor in the Department of Chemical and Materials Engineering at the University of Alberta. He has published more than 100 articles in peer-reviewed journals and is the editor of Engineered Carbohydrate-Based Materials for Biomedical Applications (Wiley). Dr. Narain’s research examines nanomedicine and regenerative medicine, with an emphasis on developing new biomaterials for gene delivery and tissue engineering.

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• PREFACE viiCONTRIBUTORS ixSECTION I GENERAL METHODS OF BIOCONJUGATION1 Covalent and Noncovalent Bioconjugation Strategies 3Rajesh Sunasee and Ravin NarainSECTION II POLYMER BIOCONJUGATES2 Bioconjugates Based on Poly(Ethylene Glycol)s and Polyglycerols 79Rajesh A. Shenoi, Fang Gao, Muhammad Imran ul-Haq, and Jayachandran N. Kizhakkedathu3 Synthetic Polymer Bioconjugate Systems 104Marya Ahmed and Ravin Narain4 Natural Polymer Bioconjugate Systems 133Lichao Liu, Xiuwei Pan, and Weian Zhang5 Dendrimer Bioconjugates: Synthesis and Applications 146Ali Nazemi and Elizabeth R. GilliesSECTION III ORGANIC NANOPARTICLES BASED BIOCONJUGATES6 Bioconjugation Strategies: Lipids, Liposomes, Polymersomes, and Microbubbles 187Anirban Sen Gupta and Horst A. von Recum7 Organic Nanoparticle Bioconjugate: Micelles, Cross-Linked Micelles, and Nanogels 203Maria Vamvakaki8 Carbon Nanotubes and Fullerene C60 Bioconjugates 239Keshwaree Babooram and Ravin NarainSECTION IV INORGANIC NANOMATERIALS BIOCONJUGATES (METALS, METAL OXIDES—QUANTUM DOTS, IRON-OXIDE)9 Gold Nanomaterials Bioconjugates 257Qian Yang10 Methods for Magnetic Nanoparticle Synthesis and Functionalization 281Mohammad H. El-Dakdouki, Kheireddine El-Boubbou, Jingguang Xia, Herbert Kavunja, and Xuefei Huang11 Quantum Dots Bioconjugates 315Xiaoze Jiang, Meifang Zhu, and Ravin Narain12 Silica Nanoparticle Bioconjugates 327Yohei Kotsuchibashi, Mitsuhiro Ebara, and Ravin Narain13 Polyhedral Oligomeric Silsesquioxanes (POSS) Bioconjugates 344Weian Zhang, Zhenghe Zhang, and Lizhi HongSECTION V CELL-BASED, HYDROGELS/MICROGELS AND GLYCO-BIOCONJUGATES14 Cell-Based Bioconjugates 357Mitsuhiro Ebara15 Bioresponsive Hydrogels and Microgels 370Xue Li and Michael J. Serpe16 Conjugation Strategies Used for the Preparation of Carbohydrate-Conjugate Vaccines 387Rachel Hevey and Chang-Chun LingSECTION VI CHARACTERIZATION, PHYSICO-(BIO)CHEMICAL PROPERTIES, AND APPLICATIONS OF BIOCONJUGATES17 Properties and Characterization of Bioconjugates 417Ali Faghihnejad, Jun Huang, and Hongbo Zeng18 Physico-Chemical and Biochemical Properties of Bioconjugates 431Marya Ahmed and Ravin Narain19 Applications of Bioconjugates 444Marya Ahmed and Ravin NarainINDEX 457

RAVIN NARAIN, PhD, is Associate Professor in the Department of Chemical and Materials Engineering at the University of Alberta. He has published more than 100 articles in peer-reviewed journals and is the editor of Engineered Carbohydrate-Based Materials for Biomedical Applications (Wiley). Dr. Narain’s research examines nanomedicine and regenerative medicine, with an emphasis on developing new biomaterials for gene delivery and tissue engineering.

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• PREFACE viiCONTRIBUTORS ixSECTION I GENERAL METHODS OF BIOCONJUGATION1 Covalent and Noncovalent Bioconjugation Strategies 3Rajesh Sunasee and Ravin NarainSECTION II POLYMER BIOCONJUGATES2 Bioconjugates Based on Poly(Ethylene Glycol)s and Polyglycerols 79Rajesh A. Shenoi, Fang Gao, Muhammad Imran ul-Haq, and Jayachandran N. Kizhakkedathu3 Synthetic Polymer Bioconjugate Systems 104Marya Ahmed and Ravin Narain4 Natural Polymer Bioconjugate Systems 133Lichao Liu, Xiuwei Pan, and Weian Zhang5 Dendrimer Bioconjugates: Synthesis and Applications 146Ali Nazemi and Elizabeth R. GilliesSECTION III ORGANIC NANOPARTICLES BASED BIOCONJUGATES6 Bioconjugation Strategies: Lipids, Liposomes, Polymersomes, and Microbubbles 187Anirban Sen Gupta and Horst A. von Recum7 Organic Nanoparticle Bioconjugate: Micelles, Cross-Linked Micelles, and Nanogels 203Maria Vamvakaki8 Carbon Nanotubes and Fullerene C60 Bioconjugates 239Keshwaree Babooram and Ravin NarainSECTION IV INORGANIC NANOMATERIALS BIOCONJUGATES (METALS, METAL OXIDES—QUANTUM DOTS, IRON-OXIDE)9 Gold Nanomaterials Bioconjugates 257Qian Yang10 Methods for Magnetic Nanoparticle Synthesis and Functionalization 281Mohammad H. El-Dakdouki, Kheireddine El-Boubbou, Jingguang Xia, Herbert Kavunja, and Xuefei Huang11 Quantum Dots Bioconjugates 315Xiaoze Jiang, Meifang Zhu, and Ravin Narain12 Silica Nanoparticle Bioconjugates 327Yohei Kotsuchibashi, Mitsuhiro Ebara, and Ravin Narain13 Polyhedral Oligomeric Silsesquioxanes (POSS) Bioconjugates 344Weian Zhang, Zhenghe Zhang, and Lizhi HongSECTION V CELL-BASED, HYDROGELS/MICROGELS AND GLYCO-BIOCONJUGATES14 Cell-Based Bioconjugates 357Mitsuhiro Ebara15 Bioresponsive Hydrogels and Microgels 370Xue Li and Michael J. Serpe16 Conjugation Strategies Used for the Preparation of Carbohydrate-Conjugate Vaccines 387Rachel Hevey and Chang-Chun LingSECTION VI CHARACTERIZATION, PHYSICO-(BIO)CHEMICAL PROPERTIES, AND APPLICATIONS OF BIOCONJUGATES17 Properties and Characterization of Bioconjugates 417Ali Faghihnejad, Jun Huang, and Hongbo Zeng18 Physico-Chemical and Biochemical Properties of Bioconjugates 431Marya Ahmed and Ravin Narain19 Applications of Bioconjugates 444Marya Ahmed and Ravin NarainINDEX 457

RAVIN NARAIN, PhD, is Associate Professor in the Department of Chemical and Materials Engineering at the University of Alberta. He has published more than 100 articles in peer-reviewed journals and is the editor of Engineered Carbohydrate-Based Materials for Biomedical Applications (Wiley). Dr. Narain’s research examines nanomedicine and regenerative medicine, with an emphasis on developing new biomaterials for gene delivery and tissue engineering.

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• PREFACE viiCONTRIBUTORS ixSECTION I GENERAL METHODS OF BIOCONJUGATION1 Covalent and Noncovalent Bioconjugation Strategies 3Rajesh Sunasee and Ravin NarainSECTION II POLYMER BIOCONJUGATES2 Bioconjugates Based on Poly(Ethylene Glycol)s and Polyglycerols 79Rajesh A. Shenoi, Fang Gao, Muhammad Imran ul-Haq, and Jayachandran N. Kizhakkedathu3 Synthetic Polymer Bioconjugate Systems 104Marya Ahmed and Ravin Narain4 Natural Polymer Bioconjugate Systems 133Lichao Liu, Xiuwei Pan, and Weian Zhang5 Dendrimer Bioconjugates: Synthesis and Applications 146Ali Nazemi and Elizabeth R. GilliesSECTION III ORGANIC NANOPARTICLES BASED BIOCONJUGATES6 Bioconjugation Strategies: Lipids, Liposomes, Polymersomes, and Microbubbles 187Anirban Sen Gupta and Horst A. von Recum7 Organic Nanoparticle Bioconjugate: Micelles, Cross-Linked Micelles, and Nanogels 203Maria Vamvakaki8 Carbon Nanotubes and Fullerene C60 Bioconjugates 239Keshwaree Babooram and Ravin NarainSECTION IV INORGANIC NANOMATERIALS BIOCONJUGATES (METALS, METAL OXIDES—QUANTUM DOTS, IRON-OXIDE)9 Gold Nanomaterials Bioconjugates 257Qian Yang10 Methods for Magnetic Nanoparticle Synthesis and Functionalization 281Mohammad H. El-Dakdouki, Kheireddine El-Boubbou, Jingguang Xia, Herbert Kavunja, and Xuefei Huang11 Quantum Dots Bioconjugates 315Xiaoze Jiang, Meifang Zhu, and Ravin Narain12 Silica Nanoparticle Bioconjugates 327Yohei Kotsuchibashi, Mitsuhiro Ebara, and Ravin Narain13 Polyhedral Oligomeric Silsesquioxanes (POSS) Bioconjugates 344Weian Zhang, Zhenghe Zhang, and Lizhi HongSECTION V CELL-BASED, HYDROGELS/MICROGELS AND GLYCO-BIOCONJUGATES14 Cell-Based Bioconjugates 357Mitsuhiro Ebara15 Bioresponsive Hydrogels and Microgels 370Xue Li and Michael J. Serpe16 Conjugation Strategies Used for the Preparation of Carbohydrate-Conjugate Vaccines 387Rachel Hevey and Chang-Chun LingSECTION VI CHARACTERIZATION, PHYSICO-(BIO)CHEMICAL PROPERTIES, AND APPLICATIONS OF BIOCONJUGATES17 Properties and Characterization of Bioconjugates 417Ali Faghihnejad, Jun Huang, and Hongbo Zeng18 Physico-Chemical and Biochemical Properties of Bioconjugates 431Marya Ahmed and Ravin Narain19 Applications of Bioconjugates 444Marya Ahmed and Ravin NarainINDEX 457