Isolation and Purification of Proteins

Rajni Hatti-Kaul is Senior Lecturer, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden. The author or coauthor of 60 scientific papers, several book chapters, and two books, she received the M.Sc. (1979) and the Ph.D. (1984) degrees from the University of Bombay, India. Bo Mattiasson is Professor, Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden. The author or coauthor of more than 550 professional publications and five books, he received the M.Sc. (1971) and Ph.D. (1974) degrees from Lund University, Sweden.

• Preface, Contributors, Introduction, 1. Release of Protein from Biological Host, 2. Chromatography: The High-Resolution Technique for Protein Separation,  3. Selectivity in Affinity Chromatography, 4. Genetic Approaches to Facilitate Protein Purification, 5. Solid-Liquid Separation, 6. Membranes for Protein Isolation and Purification, 7. Precipitation of Proteins: Nonspecific and Specific, 8. Protein Crystallization for Large-Scale Bioseparation, 9. Extraction for Rapid Protein Isolation, 10. Adsorption as an Initial Step for the Capture of Proteins, 11. Fast Chromatography of Proteins, 12. Novel Approaches to the Chromatography of Proteins, 13. Scale-up and Commercial Production of Recombinant Proteins, 14. Process Monitoring During Protein Purification, 15. Protein Formulation, 16. Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purification, Index