Bioanalytical Techniques

Inamuddin, PhD, is an assistant professor at the Department of Applied Chemistry, Zakir Husain College of Engineering and Technology, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, India. He has extensive research experience in multidisciplinary fields of analytical chemistry, materials chemistry, electrochemistry, renewable energy, and environmental science. He has worked on different research projects funded by various government agencies and universities and is the recipient of awards, including the Department of Science and Technology, India, Fast-Track Young Scientist Award and Young Researcher of the Year Award 2020 from Aligarh Muslim University. He has published about 210 research articles in various international scientific journals, many book chapters, and dozens of edited books, many with Wiley-Scrivener. Tariq Altalhi, PhD, is an associate professor in the Department of Chemistry at Taif University, Saudi Arabia. He received his doctorate degree from the University of Adelaide, Australia in 2014 with Dean’s Commendation for Doctoral Thesis Excellence. He has worked as head of the Chemistry Department at Taif University and Vice Dean of Science College. In 2015, one of his works was nominated for Green Tech awards from Germany, Europe’s largest environmental and business prize. He has also co-edited a number of scientific books. Abeer Alosaimi, PhD, is an assistant professor in the Department of Chemistry at Taif University, Saudi Arabia with over 22 years of academic experience. He has over 50 publications, including one book and articles in international journals and conferences. His research interests include polymer science, nanocomposites, and organic chemistry. Jorddy Neves Cruz is a researcher at the Federal University of Pará and the Emilio Goeldi Museum, Brazil. He has experience in multidisciplinary research in the areas of medicinal chemistry, drug design, extraction of bioactive compounds, extraction of essential oils, food chemistry and biological testing. He has published several research articles in scientific journals and is an associate editor of the Journal of Medicine.

• Preface xxix1 Point-of-Care Diagnostics for Rapid Disease Detection 1Nabil H. Bhuiyan and M. Khalid Hossain2 Principles of Molecular Biology for Bioanalysis 39Syed Ali Raza Naqvi, Syeda Marab Saleem, Tania Jabbar, Atta Ul Haq, Muhammad Asif, Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Ali Abbas3 UV–Visible Spectroscopy for Quantitative Analysis of Biomolecules 79Syeda Marab Saleem, Syed Ali Raza Naqvi, Muhammad Ramzan Saeed Ashraf Janjua, Tauqir A. Sherazi, Muhammad Saeed, Sadaf Ul Hassan, Muhammad Imran, Muhammad Asif, Mamoon Ur Rasheed and Tania Jabbar4 Emerging Trends and Future Perspectives in Bioanalysis 119Jayapal Reddy Gangadi, Kalpana Swain and Satyanarayan Pattnaik5 Introduction to Biosensors for Point]of-Care Testing: IoT-Based Vitamin D3 Biosensor 137Asif Hussain Shaik and Chinna Babu Jyothi6 Fluorescence Spectroscopy for Protein–Protein Interactions 149Masoud Shayegan and Hossein Ahmadzadeh7 Flow Cytometry for Cell Analysis and Sorting 173Maryam Davardoostmanesh and Hossein Ahmadzadeh8 Application in Drug Discovery and Development for Lead Identification 189Sareena Ehsan, Rida Mumtaz, Abdul Rehman, Maryam, Habibullah Nadeem, Ammara Riaz, Jallat Khan, Ebrahim Alinia-Ahandani and Zeliha Selamoglu9 Immunoblotting Techniques for Protein Identification and Quantification and Its Application 203Megha Sikder, Somen Debnath and Biswanath Bhunia10 Gel Electrophoresis for DNA Fragment Analysis 221Aneela Nawaz, Shahzar Khan, Momina Aamir, Sidra Zaheer, Sabeena Zafar, Nauman Ahmed Khalid and Samiullah Khan11 Omics Approaches in Bioanalysis for Systems Biology Studies 239Rana Hooshang, Hoda Nouri and Hamid Moghimi12 Introduction to Mass Spectrometry for Metabolite Profiling 283Jackie E. Wood and Brendon D. Gill13 Infections, Symptoms, and Clinical Diagnostic Techniques for Dengue: A Case Study of a Neglected Tropical Disease 341Shreeganesh Subraya Hegde and Badekai Ramachandra Bhat14 Microfluidic-Based Bioassays for High-Throughput Analysis 355Muthui Martin Mwaurah, J. Mathiyarasu and A.M. Vinu Mohan15 Circular Dichroism Spectroscopy for Secondary Structure Analysis of Proteins 383Masooma Siddiqui, Maroof Ali, Mehak Sagheer and Mohd Imran Ahamed16 Optical Biosensors for Detection of Analytes in Biological Samples 427Uzma, Masooma Siddiqui and Mehtab Parveen17 Tandem Mass Spectrometry for Peptide Sequencing 461Satyendra Mishra and Neha V. Rathod18 Unraveling Complexity: Cutting-Edge Ion Chromatography Methods for the Analysis of Inorganic Ions in Biological Samples 489Muhammad Hafiznur Yunus, Nor Ain Shahera Khairi, Azren Aida Asmawi, Fatin Nabilah Mohd Faudzi, Ahmad Farabi Mohamad Saman, Nor Azah Yusof and Siti Fatimah Nur Abdul Aziz19 Sample Preparation and Handling for Reliable Results 551Virender, Rohit, Ashwani Kumar, Rakesh Kumar Gupta and Brij Mohan20 Data Analysis in Bioanalysis for Interpretation of Experimental Data 575Jaymin Parikh, Keyur Bhatt, Krunal Modi, Jasbir Sangwan, Alexander A. Solovev and Brij Mohan21 Protein Identification Using Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry 605Vipul D. Prajapati and Princy Shrivastav22 Applications of Electrothermal Atomic Absorption Spectrometry (ET-AAS) Toward Various Metal Analyses in Diverse Biological and Environmental Matrices 629Aruna Jyothi Kora23 Thin-Layer Chromatography for Natural Product Analysis 645Mahreen Imam, Syed Ali Raza Naqvi, Majid Muneer, Atta Ul Haq, Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Syeda Marab SaleemIndex 667