This text emphasizes a multidisciplinary approach combining the experiences of a nuclear medicine physician, a surgeon, and a pathologist. It should also prove an important reference for researchers and clinicians who want to become familiar with sentinel lymph node mapping. The underlying thesis in solid tumour biology is that metastasis in general starts in an orderly progression with lymphatic spread first to the sentinel lymph node (SLN) in the nearest lymph node basin. Therefore, the logical approach is to harvest that specific SLN for thorough analysis.