Principles of Gene Manipulation and Genomics

Sandy Primrose was an academic for 10 years and a senior manager in the biotechnology industry for 20 years. He now manages a consultancy practice focused on technology management.Richard Twyman worked as a research scientist at the University of Warwick and at the MRC Laboratory of Molecular Biology in Cambridge before taking up scientific writing as a full time occupation in 1998. He is currently a Visiting Fellow at the Department of Biology, University of York, and a freelance scientific writer and teacher.

"Principles of Gene Manipulation and Genomics is well written and clearly illustrated throughout, as would be expected from these authors who have considerable experience in this subject."Genetical Research

• Preface. 1. Gene Manipulation in the Post-Genomics Era.Part I: Fundamental Techniques of Gene Manipulation:.2. Basic Techniques.3. Cutting and Joining DNA Molecules.4. Basic Biology of Plasmid and Phage Vectors.5. Cosmids, Phasmids and Other Advanced Vectors.6. Gene Cloning Strategies.7. Sequencing Genes and Short Stretches of DNA.8. Changing Genes: Site-directed Mutagenesis and Protein Engineering.9. Bioinformatics.Part II: Manipulating DNA in Microbes, Plants and Animals:.10. Cloning in Bacteria Other Than Escherichia coli.11. Cloning in Saccharomyces cerevisiaea and Other Fungi.12. Gene Transfer to Animal Cells.13. Genetic Manipulation of Animals.14. Gene Transfer to Plants.15. Advanced Transgenic Technology.Part III: Genome Analysis, Genomics and Beyond:.16. The Organization and Structure of Genomes.17. Mapping and Sequencing Genomes.18. Comparative Genomics.19. Large-Scale Mutagenesis and Interference.20. Analysis of the Transcriptome.21. Proteomics I – Expression Analysis and Characterization of Proteins.22. Proteomics II – Analysis of Protein Structures.23. Proteomics III – Protein Interactions.24. Metabolomics and Global Biochemical Networks.Part IV: Applications of Gene Manipulation and Genomics:.25. Applications of Genomics: Understanding the Basis of Polygenic Disorders and Identifying Quantitative Trait Loci.26. Applications of Recombinant DNA Technology.Index