Laboratory Hematology Practice

Dr Kandice Kottke-Marchant, MD PhD, Section Head, Hemostasis and Thrombosis, Department of Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio, USA and President, International Society of Laboratory Hematology. She is also a Professor of Pathology at the Cleveland Clinic Lerner College of Medicine and an Adjunct Professor of Biomedical Engineering at Case Western Reserve University. She has published over 100 peer-reviewed publications in the field of haemostasis, is an international expert on platelet testing and aspirin resistance, and has served on review panels from the National Institutes of Health (NIH), College of American Pathologists (CAP), ISO/TC212 and North American Specialized Coagulation Laboratory Association (NASCOLA). She has been a driving force to increase the international visibility and impact of the ISLH. She has been responsible for running the ISLH annual Symposia since 2002. Due to her involvement in haemostasis and the ISLH, Dr. Kottke-Marchant will be uniquely positioned to serve as one of the co-Editors of the Textbook of Laboratory Hematology. Elkin Simson, MD, PCAP, FACSP is the Medical Director of the Center for Clinical Laboratories and Hematology Laboratory at the Mount Sinai Hospital. He is also Associate Professor and Vice Chairman of the Department of Pathology at the Mount Sinai School of Medicine, New York, NY, USA. Dr. Simson was formerly the Vice-President for Medical and Scientific Affairs at Technicon Instruments Corporation, where he participated in the development of the first automated haematology analyzers to combine the CBC and WBC differential into a single instrument. Dr Simson is the Past President of the ISLH and is Vice-President of the International Council for Standardization in Hematology (ICSH). He has been Chairman of the Hematology Area Committee of NCCLS (now CLSI), a member of Council of the National Reference System for the Clinical Laboratory (NRSCL) and the Hematology and Quality Improvement Subcommittees of the NCCCLS. He was a member of the Hematology and Pathology Advisory Panel of the Food and Drug Administration (FDA). Due to his long-time involvement in haematology automation and standards organizations, Dr. Simson is an idea co-editor.The proposed section editors are chosen from international experts in their respective fields; most have also been long-time ISLH members who have been faculty at one or more ISLH Symposia and who have been repeatedly recognized as outstanding speakers and contributors by the participants. Dr Bruce Davis is one of the co-founders of the ISLH and has a long history of involvement in developing automated cellular and flow cytometric technologies. Professor Alberto Orfao from Spain is one of the world’s foremost experts on flow cytometry. Professor Samuel Machin is currently President of the British Society of Haematology and is an international authority on haemostasis testing. Stefanie McFadden is a medical technologist who is sought after as a consultant for laboratory management and informatics issues.

“This is an interesting book for those with a primary interest in laboratory hematology.”  (Doody’s, 7 September 2012)

• Contributors, viii Preface, xiiiAbbreviations, xvI Cellular Analysis, 11 Historical Perspective on Cellular Analysis, 3Elkin Simson2 Cellular Morphologic Analysis of Peripheral Blood, 10Powers Peterson, Sheila McNeill, and Gene Gulati3 Automated Cell Analysis: Principles, 26Bruce H. Davis and Patrick W. Barnes4 Differential Leukocyte Analysis, 33Marie-Christine Béné and Francis Lacombe5 Automated Platelet Analysis, 48Carol Briggs and Samuel J. Machin6 Method Standardization in Cellular Analysis, 59Ian Giles & Richard Kendall7 Spurious counts and spurious results on hematology analyzers: platelets, 66Marc Zandecki, Franck Genevieve, Jérémie Gérard, and Alban Godon8 Spurious Counts and Spurious Results on Hematology Analyzers: White Blood Cells, Red Blood Cells, Hemoglobin, Red Cell Indices, and Reticulocytes, 79Marc Zandecki, Franck Genevieve, Jérémie Gérard, and Alban Godon9 Evaluation of Hematology Analyzers, 96Carol BriggsII Flow Cytometry in Hematologic Diagnosis, 10310 Flow Cytometric Specimen Collection, Processing, and Reporting, 105Maryalice Stetler-Stevenson, Bruce Greig, Constance Yuan11 Validation and Quality Control in Clinical Flow Cytometry, 115Norman B. Purvis and Teri Oldaker12 A New Paradigm for Cytometric Analysis, 131C. Bruce BagwellIII Molecular Diagnostics, 14313 Sample Collection, Processing, and Storage for Molecular Genetic Testing, 145Bobby L. Boyanton Jr. and Domnita Crisan14 Development of Amplification-Based Molecular Genetic Testing in Hematology, 155Raymond R. Tubbs15 Single Nucleotide Polymorphisms in Molecular Diagnostics, 168Gurunathan Murugesan, Seongsoo Jang, and Jin-Yeong Han16 Molecular Cytogenetic (FISH) Analysis of Hematolymphoid Disorders, 181Mark A. Micale and Anwar N. Mohamed17 Immunoglobulin and T-Cell Receptor Gene Rearrangement Analysis for Diagnosis of Hematologic Malignancies, 199Jacques J. M. van Dongen, Anton W. Langerak, and Tomasz Szczepan ski18 Minimal Residual Disease Detection in Acute Myeloid Leukemia by RQ-PCR and Multiparameter Flow Cytometry, 220Wolfgang Kern and Susanne Schnittger19 Engraftment Analysis using Short Tandem Repeats following Allogeneic Hematopoietic Cell Transplantation, 234Ilka Warshawsky and Hyun-Sook Chi20 Molecular Profiling Methods in the Diagnosis of Hematologic Disorders, 244Annette S. Kim, Stephen R. Master, and Cherie H. Dunphy21 The Role of Classical Cytogenetics in Hematologic Diagnosis, 267Kathleen RichkindIV Bone Marrow Analysis, 27922 Bone Marrow Processing and Normal Morphology, 281Karl S. Theil23 Cytochemical, Immunocytochemical, Histochemical, and Immunohistochemical Staining of Bone Marrow and Peripheral Blood, 300Alexandra Rice and Barbara J. Bain24 The Myeloproliferative Neoplasms, 315Sindhu Cherian and Brent L. Wood25 Myelodysplastic Disorders, 331Maryalice Stetler-Stevenson, Joseph A. DiGiuseppe, and Diane C. Arthur26 Acute Leukemias, 345Joseph A. DiGiuseppe and Laila Mnayer27 Lymphoproliferative Disorders, 364Robert W. Allan, Samer Z. Al-Quran, Ying Li, and Raul C. Braylan28 Plasma Cell Disorders, 381Bill G. Richendollar and James R. Cook29 Diagnosis of Adult Mastocytosis: Role for Bone Marrow Analysis, 388Luis Escribano, Andrés Garcia-Montero, Laura Sanchez-Muñoz, Cristina Teodosio, Ivan Alvarez-Twose, Maria Jara-Acevedo, José Mario Morgado, Julia Almeida, Alberto Orfao for the Spanish Network on Mastocytosis (REMA)30 Paroxysmal Nocturnal Hemoglobinuria (PNH) Analysis, 399Stephen J. RichardsV Hemostasis and Thrombosis, 40731 Sample Collection and Processing in Hemostasis, 409Dorothy M. Adcock32 Performance and Interpretation of Routine Coagulation Assays, 420Kandice Kottke-Marchant33 Clotting Factors and Inhibitors: Assays and Interpretation, 435Piet Meijer, H. W. Verbruggen, and Michael Spannagl34 Diagnosis of von Willebrand Disease, 447Emmanuel J. Favaloro and Jerry Koutts35 Diagnosis of Fibrinolytic Disorders, 460Wayne Chandler36 Laboratory Evaluation of Acquired Coagulation Disorders, 468Richard A. Marlar and Katerine Seywerd37 Platelet Assays and Platelet Dysfunction, 480Paul Harrison and David Keeling38 Thrombophilia: Assays and Interpretation, 492Sophia Yohe and John Olson39 Lupus Anticoagulants, Antiphospholipid Antibodies, and Antiphospholipid Syndrome, 509William L. Nichols, Kandice Kottke-Marchant, Marlies R. Ledford-Kraemer, Henry A. Homburger, Layna K. Cardel40 Heparin-Induced Thrombocytopenia, 526Sixten Selleng and Andreas Greinacher41 Anticoagulant, Antiplatelet, and Thrombolytic Drugs, 535Laura Green and Samuel J. Machin42 Guidelines for Evaluation of Coagulation Analyzers and Coagulation Testing, 543Chris GardinerVI Special Hematology Techniques, 55343 Essentials of Immunohematology, 555A. Bradley Eisenbrey44 Analysis of Hemoglobinopathies, Hemoglobin Variants and Thalassemias, 562Monica V. E. Gallivan and Piero C. Giordano45 Nutritional Anemia, 586Ralph Green46 Hemolytic Anemias Associated with Disorders of Erythrocyte Membrane and Cytoskeleton Proteins, 602May-Jean King, Lesley J. Bruce, Didier Dhermy47 Body Fluid Analysis, 615David J. Blomberg, Katherine Galagan, and Eric F. Glassy48 Malaria Analysis, 626Qigui Li, Peter J. Weina, and R. Scott Miller49 Erythrocyte Sedimentation Rate (ESR), 638Josep M. JouVII Point-of-Care Testing, 64750 Needs Analysis and Selection of Point-of-Care Testing Analyzers, 649Albert Huisman51 Point-of-Care Testing in Hemostasis, 655Steve Kitchen and Dianne Kitchen52 Point-of-Care Hematology Assays, 668Albert HuismanVIII Management of Information 67353 Laboratory Management, 675Stefanie L. McFadden and Linda M. Sandhaus54 Hematology Quality Practices 686George S. Cembrowski55 Digital Imaging in Hematology, 707Danny Hsu and Szu-Hee LeeIndex, 719