Clinical Embryology

Zsolt Peter Nagy, MD, PhD, HCLD (ABB), EMB (ACE), Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, GA, USA Alex C. Varghese, PhD, Senior Embryologist, Montreal Reproductive Centre, Montreal, QC, Canada Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

• Journey of Human Gametes In Vitro: 1978 to 2010.- Part I: Sperm Processing Methods.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Part II: Embryo Culture Methods.- Culture Media in IVF: Decisions for the Laboratory.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Part III: Insemination Procedures.- Intrauterine Insemination.- Conventional IVF Insemination.- Oocyte Denuding.- Intracytoplasmic Sperm Injection.- Part IV: Embryo Evaluation and Grading.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metablomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-Time Embryo Monitoring Devices for Embryo Selection.- Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis (Eeva Test).- Predicting Embryo Implantation Potential Using Video Monitoring by the Embryoscope™ Time-Lapse System.- Part V: Genetic Testing of Embryos.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosomal Abnormalities and Gene Defects.- Part VI: Cryopreservation.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Part VII: Embryo Transfer.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-BasedEvaluation.