Zsolt Peter Nagy – författare

For around half of the couples who have trouble conceiving the cause of infertility is sperm-related. Intracytoplasmic sperm injection (ICSI) is the most common and successful treatment for male infertility. Here, the pioneers for the technique, along with authorities in the field, describe the underlying science of ICSI and other micromanipulation techniques. Practical advice for performing the techniques is covered in depth, including sperm selection, laser-assisted ICSI, and the use of piezo in ICSI. Examining the safety of ICSI in animal models as well as the impact of ICSI on the health and well-being of the children conceived through the procedure is discussed. This manual is an essential resource for clinical embryologists and laboratory personnel wishing to refine or develop techniques and improve outcomes.

​This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Gamete Assessment, Selection and Micromanipulation in ART covers a variety of topics, including: - Human oocyte evaluation - Sperm evaluation and selection - Micromanipulators and micromanipulation - Biopsy procedures on oocytes and embryos - Molecular insights in IVF Practical for both clinicians and researchers alike, Gamete Assessment, Selection and Micromanipulation in ART brings together all of the need-to-know information about these important topics in reproductive medicine.

Building and Managing an IVF Laboratory

This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Clinical Embryology covers a variety of topics, including: - Sperm processing methods - Embryo culture methods - Insemination procedures - Embryo evaluation - Genetic testing of embryos - Cryopreservation - Embryo transfer Also included are two new chapters on predicting embryo developmental potential and viability using automated time-lapse analysis (Eeva) and predicting embryo implantation potential using Embryoscope.™ Practical for both clinicians and researchers alike, Clinical Embryology brings together all of the need-to-know information about these important topics in reproductive medicine.

The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods andProtocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods andProtocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.

Cryopreservation of oocytes, sperm, and ovarian and testicular tissues, as well as embryos, is one of the most critical procedures to preserve the reproductive capacity of individuals. It is an indispensable part of assisted reproductive technologies, as nearly all IVF clinics around the world have embraced the freeze-all embryo strategy with no fresh embryo transfers. Advanced platforms, such as automation and artificial intelligence, are making their way into all aspects of assisted reproductive technologies, including reproductive tissue banking process and storage. At the same time, lax regulations and lack of training combined with rapid demands of IVF services have resulted in a climate of frequent disaster and catastrophic incidents from the cryo labs that store thousands of patients’ embryos for years. With the onset of malpractice lawsuits against the clinics and awards of large compensation to the patients, regulations are getting stricter in this arena to safeguard the integrity of storage systems, and industry leaders are developing advanced devices and alarm systems to remotely monitor storage systems exploring the power of internet, AI and automation. Therefore, there is an urgent need for a comprehensive text in this field based on the introduction of such a wide array of advanced devices, newer technologies, regulatory frameworks, risks and disaster management options.With contributions from top internationally recognized scientists and clinicians with expertise in cryopreservation and reproductive technology, this book provides a comprehensive overview of the basics of cryobiological processes and a technically detailed presentation on all aspects of cryopreservation of reproductive cells and tissues. It presents the current, well-established procedures, as well as novel techniques with the latest innovations described in detail.Bringing together the latest information with key thought leaders in the field, Cryopreservation in Assisted Reproduction is intended to be the go-to resource for all reproductive medicine clinicians, embryologists, lab technologists, IVF lab directors/managers, and researchers.

Cryopreservation of oocytes, sperm, and ovarian and testicular tissues, as well as embryos, is one of the most critical procedures to preserve the reproductive capacity of individuals. It is an indispensable part of assisted reproductive technologies, as nearly all IVF clinics around the world have embraced the freeze-all embryo strategy with no fresh embryo transfers. Advanced platforms, such as automation and artificial intelligence, are making their way into all aspects of assisted reproductive technologies, including reproductive tissue banking process and storage. At the same time, lax regulations and lack of training combined with rapid demands of IVF services have resulted in a climate of frequent disaster and catastrophic incidents from the cryo labs that store thousands of patients’ embryos for years. With the onset of malpractice lawsuits against the clinics and awards of large compensation to the patients, regulations are getting stricter in this arena to safeguard the integrity of storage systems, and industry leaders are developing advanced devices and alarm systems to remotely monitor storage systems exploring the power of internet, AI and automation. Therefore, there is an urgent need for a comprehensive text in this field based on the introduction of such a wide array of advanced devices, newer technologies, regulatory frameworks, risks and disaster management options.With contributions from top internationally recognized scientists and clinicians with expertise in cryopreservation and reproductive technology, this book provides a comprehensive overview of the basics of cryobiological processes and a technically detailed presentation on all aspects of cryopreservation of reproductive cells and tissues. It presents the current, well-established procedures, as well as novel techniques with the latest innovations described in detail.Bringing together the latest information with key thought leaders in the field, Cryopreservation in Assisted Reproduction is intended to be the go-to resource for all reproductive medicine clinicians, embryologists, lab technologists, IVF lab directors/managers, and researchers.