Zsolt Peter Nagy, MD, PhD, Scientific Director, Reproductive Biology Associates – Prelude, Atlanta, GA, USA - Alex C. Varghese, Group Scientific Director, Fertility Associates Auckland, New Zealand , Sunfert International, Malaysia Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director of Research, Global Andrology Forum, Moreland Hills, and Emeritus Staff, Cleveland Clinic, Cleveland, OH, USA

• PART I Introduction.- Cryopreservation in global perspectives: Editors' thoughts.- Historical Background on Gamete and Embryo Cryopreservation.- PART II Principles and Models.- Principles of Cryopreservation.- Animal Models for Human Oocyte, Embryo, and Tissue Cryopreservation.- Considerations in Immature Oocyte Cryopreservation.- Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification.- Devitrification Thresholds and Warming Rates of Reproductive Cells.- PART III Methodology and Management.- Human Sperm Slow-freezing.- Human Sperm Vitrification.- Human Epididymal and Testicular Sperm Cryopreservation.- Human Oocyte Slow-freezing.- Human Embryo (Zygote, Cleaved, Blastocysts) Slow-freezing.- Human Oocyte Vitrification.- Human Embryo Vitrification  (zygote/cleavage/blastocyst).- Human Ovarian Tissue Slow-freezing.- Human Ovarian Tissue Vitrification.-Vitrification: implementation and training.- Establishing a Donor Oocyte Cryo Bank Network:  USA Experience.- Establishing a Donor Oocyte Cryo Bank Network: European Experience.- Managing a Sperm Bank: European Experience.- Managing a Sperm Bank: USA Experience.- Managing a central cryo depository.- PART IV Advances in Cryotechnology, Research, and Animal Models.- Artificial Intelligence (AI) and oocyte/embryo assessment in cryopreservation cycles.- Vitrification: A Reliable Method for Cryopreservation of Animal Embryos.- Cryopreservation Effect on Genetic/Epigenetic Function: Neonatal Outcomes.- Fertility Preservation and Genome Resource Banking for Rare and Endangered Animal Species.- End of Ice Age and the beginning of Space Age: The Freeze drying of human gametes.- PART V Expected outcomes and Quality Control.- Quality control and KPIs in cryopreservation: Aspiring for the best results.- Handling and assessing of human oocytes and blastocysts after vitrification and warming.- Health of offspring following cryopreservation of oocytes and embryos.- Elective and Onco-fertility preservation outcomes after oocyte vitrification.- PART VI Multiple vitrification-warming and biopsy procedures on human Embryos.- Fertility preservation in pre-pubertal girls.- Fertility Preservation in Transgender Patients.- Fertility Preservation in pre-adolescent and adolescent males: Recent Developments and New Insights.- PART VII Guidelines, Regulations and Best Practices.- Setting up a cryopreservation lab and selecting media, disposables and systems for vitrification.- Cryopreservation and storage of reproductive tissues: Good practice guidelines.- Transportation of Frozen Gametes and Embryos- Good Practice Guidelines.- Good practice guideline of ovarian tissue transportation for optimizing fertility outcomes.- Disposition of cryopreserved specimen.- European regulations for Reproductive Tissue banks.- Ethical issues related to cryopreserved reproductive specimen.- Legal liability landscape and the person/property divide.- Challenges on safe keeping cryo stored specimen in time of armed conflict: Live observations from the war zone.- PART VIII Industrial breakthroughs in cryopreservation and cryo-storage.- At home sperm banking kits.- Single sperm cryopreservation for azoospermia management.- Keeping cryopreserved specimen safe: Thermographic Imaging.- Robotic cryostorage solution.- Maximizing Donor Egg Efficiency - Artificial Intelligence & Genetically Certified Oocytes.- Automating Vitrification.- EmbryoOptions.- LN2 Sterilization to avoid cross contamination.- Lab on a chip – Cryopreservation.- PART IX Appendices – Protocols.- Oocyte slow freezing protocol.- Embryo slow freezing protocol (zygote/cleavage/blastocyst).- Human ovarian tissue slow freezing protocol.- Proper maintenance of dewars/cryo-storage tanks.0 Human Sperm Vitrification.- SOP on maintaining and managing cryopreserved samples and cryo-tanks in an IVF lab.

Zsolt Peter Nagy, MD, PhD, Scientific Director, Reproductive Biology Associates – Prelude, Atlanta, GA, USA - Alex C. Varghese, Group Scientific Director, Fertility Associates Auckland, New Zealand , Sunfert International, Malaysia Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director of Research, Global Andrology Forum, Moreland Hills, and Emeritus Staff, Cleveland Clinic, Cleveland, OH, USA

• PART I Introduction.- Cryopreservation in global perspectives: Editors' thoughts.- Historical Background on Gamete and Embryo Cryopreservation.- PART II Principles and Models.- Principles of Cryopreservation.- Animal Models for Human Oocyte, Embryo, and Tissue Cryopreservation.- Considerations in Immature Oocyte Cryopreservation.- Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification.- Devitrification Thresholds and Warming Rates of Reproductive Cells.- PART III Methodology and Management.- Human Sperm Slow-freezing.- Human Sperm Vitrification.- Human Epididymal and Testicular Sperm Cryopreservation.- Human Oocyte Slow-freezing.- Human Embryo (Zygote, Cleaved, Blastocysts) Slow-freezing.- Human Oocyte Vitrification.- Human Embryo Vitrification  (zygote/cleavage/blastocyst).- Human Ovarian Tissue Slow-freezing.- Human Ovarian Tissue Vitrification.-Vitrification: implementation and training.- Establishing a Donor Oocyte Cryo Bank Network:  USA Experience.- Establishing a Donor Oocyte Cryo Bank Network: European Experience.- Managing a Sperm Bank: European Experience.- Managing a Sperm Bank: USA Experience.- Managing a central cryo depository.- PART IV Advances in Cryotechnology, Research, and Animal Models.- Artificial Intelligence (AI) and oocyte/embryo assessment in cryopreservation cycles.- Vitrification: A Reliable Method for Cryopreservation of Animal Embryos.- Cryopreservation Effect on Genetic/Epigenetic Function: Neonatal Outcomes.- Fertility Preservation and Genome Resource Banking for Rare and Endangered Animal Species.- End of Ice Age and the beginning of Space Age: The Freeze drying of human gametes.- PART V Expected outcomes and Quality Control.- Quality control and KPIs in cryopreservation: Aspiring for the best results.- Handling and assessing of human oocytes and blastocysts after vitrification and warming.- Health of offspring following cryopreservation of oocytes and embryos.- Elective and Onco-fertility preservation outcomes after oocyte vitrification.- PART VI Multiple vitrification-warming and biopsy procedures on human Embryos.- Fertility preservation in pre-pubertal girls.- Fertility Preservation in Transgender Patients.- Fertility Preservation in pre-adolescent and adolescent males: Recent Developments and New Insights.- PART VII Guidelines, Regulations and Best Practices.- Setting up a cryopreservation lab and selecting media, disposables and systems for vitrification.- Cryopreservation and storage of reproductive tissues: Good practice guidelines.- Transportation of Frozen Gametes and Embryos- Good Practice Guidelines.- Good practice guideline of ovarian tissue transportation for optimizing fertility outcomes.- Disposition of cryopreserved specimen.- European regulations for Reproductive Tissue banks.- Ethical issues related to cryopreserved reproductive specimen.- Legal liability landscape and the person/property divide.- Challenges on safe keeping cryo stored specimen in time of armed conflict: Live observations from the war zone.- PART VIII Industrial breakthroughs in cryopreservation and cryo-storage.- At home sperm banking kits.- Single sperm cryopreservation for azoospermia management.- Keeping cryopreserved specimen safe: Thermographic Imaging.- Robotic cryostorage solution.- Maximizing Donor Egg Efficiency - Artificial Intelligence & Genetically Certified Oocytes.- Automating Vitrification.- EmbryoOptions.- LN2 Sterilization to avoid cross contamination.- Lab on a chip – Cryopreservation.- PART IX Appendices – Protocols.- Oocyte slow freezing protocol.- Embryo slow freezing protocol (zygote/cleavage/blastocyst).- Human ovarian tissue slow freezing protocol.- Proper maintenance of dewars/cryo-storage tanks.0 Human Sperm Vitrification.- SOP on maintaining and managing cryopreserved samples and cryo-tanks in an IVF lab.