Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.
