Plant Cell Culture Protocols

"... a valuable update on the technology, with emphasis on methods for micropropagation and gene transfer...the editor has assembled an excellent range of expert authors and the volume is well balanced and up-to-date...thoroughly recommended."-Plant Growth Regulation "Protocols are presented for many agronomically important plants including the culture and regeneration of rice, potato, barley, casava, spruce, Pasiflora, Lolium, and various species of Brassica. The organization of all of the chapters is consistent including an introduction, a materials section divided into appropriate subsections, a similarly subdivided methods section, a notes section that contains important observations to make or precautions to take, and a final reference section...the techniques are well written and include specific examples and step-by-step methods with appropriate references...will make an excellent addition to the library of researchers who wish to extend their collection of basic plant cell culture techniques."-Quarterly Review of Biology "The combination of many years of experience with new experimental entries is often very successful and of high quality...represents one of the highest quality manuals on this topic published with the last ten years...it will assure the relevance of an important scientific discipline, plant biotechnology, in today's world."-Biologia Plantarum "The book brings together a series of well written, step-by-step protocols on the most frequently used techniques in plant cell and tissue culture, covering various aspects of culture initiation, maintenance, long-term storage and manipulation...In addition to protocols on widely applied methods, each part includes representative protocols for more specialized techniques...All the chapters are well structured, profusely illustrated with original photographs and line drawings, and contain detailed descriptions of materials, culture media and comprehensively described experimental procedures. The reader will certainly appreciate the precautionary notes and additional hints on each technique and each protocol provided at the end of each chapter."-Elsevier "This book presents a comprehensive collection of applicable techniques for plant cell and tissue culture. The methods cover culture initiation, maintenance, manipulation, application and long-term storage, with emphasis on techniques for genetic modification and micropropagation." - Lavoisier Librairie

• An Introduction to Plant-Cell Culture.- Cell Culture and Plant Regeneration.- Callus Initiation, Maintenance, and Shoot Induction in Rice.- Callus Initiation, Maintenance, and Shoot Induction in Potato.- Somatic Embryogenesis in Barley Suspension Cultures.- Somatic Embryogenesis in Picea Suspension Cultures.- Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes.- Immature Inflorescence Culture of Cereals.- Cryopreservation of Rice Tissue Cultures.- Noncryogenic, Long-Term Germplasm Storage.- Plant Propagation In Vitro.- Micropropagation of Strawberry via Axillary Shoot Proliferation.- Meristem-Tip Culture for Propagation and Virus Elimination.- Clonal Propagation of Orchids.- In Vitro Propagation of Succulent Plants.- Micropropagation of Flower Bulbs.- Clonal Propagation of Woody Species.- Spore-Derived Axenic Cultures of Ferns as a Method of Propagation.- Applications for Plant Protoplasts.- Protoplast Isolation, Culture, and Plant Regeneration from Passiflora.- Isolation, Culture, and Plant Regeneration of Suspension-Derived Protoplasts of Lolium.- Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae.- Production of Cybrids in Rapeseed (Brassica napus).- Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines.- Guard Cell Protoplasts.- In Vitro Fertilization with Isolated Single Gametes.- Protocols for Genomic Manipulation.- Protocols for Anther and Microspore Culture of Barley.- Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco.- Embryo Rescue Following Wide Crosses.- Mutagenesis and the Selection of Resistant Mutants.- The Generation of Plastid Mutants In Vitro.- Protocols for the Introduction of Specific Genes.- Agrobacterium-Mediated Transformation of Petunia Leaf Disks.- Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts.- Transformation of Wheat via Particle Bombardment.- Plant Transformation via Protoplast Electroporation.- Transformation of Maize via Tissue Electroporation.- Transformation of Maize Using Silicon Carbide Whiskers.- Suspension Culture Initiation and the Accumulation of Metabolites.- Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures.- Alkaloid Accumulation in Catharanthus roseus Suspension Cultures.- Betalains.