Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9

• PTEN: History of a Tumor Suppressor.- PTEN at 18: Still Growing.- Immunopathologic Assessment of PTEN Expression.- Measurement of PTEN by Flow Cytometry.- Detecting PTEN and PI3K Signalling in Brain.- Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome.- Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.- Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET.- Methods for the Identification of PTEN-Targeting MicroRNAs.- Post-Transcriptional Regulation of PTEN by Competing Endogenous RNAs.- In Cell and In Vitro Assays to Measure PTEN Ubiquitination.- Assessing PTEN Subcellular Localization.- Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum.- Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry.- Methods for PTEN in Stem Cells and Cancer Stem Cells.- Modeling Cancer Associated Mutations of PTEN in Mice.- C. elegans Methods to Study PTEN.

Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9

• PTEN: History of a Tumor Suppressor.- PTEN at 18: Still Growing.- Immunopathologic Assessment of PTEN Expression.- Measurement of PTEN by Flow Cytometry.- Detecting PTEN and PI3K Signalling in Brain.- Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome.- Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.- Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET.- Methods for the Identification of PTEN-Targeting MicroRNAs.- Post-Transcriptional Regulation of PTEN by Competing Endogenous RNAs.- In Cell and In Vitro Assays to Measure PTEN Ubiquitination.- Assessing PTEN Subcellular Localization.- Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum.- Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry.- Methods for PTEN in Stem Cells and Cancer Stem Cells.- Modeling Cancer Associated Mutations of PTEN in Mice.- C. elegans Methods to Study PTEN.

Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9

• PTEN: History of a Tumor Suppressor.- PTEN at 18: Still Growing.- Immunopathologic Assessment of PTEN Expression.- Measurement of PTEN by Flow Cytometry.- Detecting PTEN and PI3K Signalling in Brain.- Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome.- Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.- Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET.- Methods for the Identification of PTEN-Targeting MicroRNAs.- Post-Transcriptional Regulation of PTEN by Competing Endogenous RNAs.- In Cell and In Vitro Assays to Measure PTEN Ubiquitination.- Assessing PTEN Subcellular Localization.- Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum.- Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry.- Methods for PTEN in Stem Cells and Cancer Stem Cells.- Modeling Cancer Associated Mutations of PTEN in Mice.- C. elegans Methods to Study PTEN.