Plant Mitochondria

Methods and Protocols

AvJames Whelan,Monika W. Murcha

Häftad, Engelska, 2017

Del i serien Methods in Molecular Biology

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Beskrivning

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities.

Produktinformation

Utgivningsdatum:2017-06-26
Mått:178 x 254 x 17 mm
Vikt:693 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:303
Förlag:Humana Press Inc.
ISBN:9781493949069

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik

Innehållsförteckning

Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa.- Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5’ End Maturation.- Mitochondrial Run-On Transcription Assay Using Biotin Labeling.- In Vitro RNA Uptake Studies in Plant Mitochondria.- In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria.- Plant Mitochondrial Proteomics.- Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites.- A Flowchart to Analyze Protease Activity in Plant Mitochondria.- Activity Measurements of Mitochondrial Enzymes in Native Gels.- Activity Assay for Plant Mitochondrial Enzymes.- Analysis of Type II NAD(P)H Dehydrogenases.- Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes.- Micro-Respiration Measurements in Plants.- Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation.- Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana.- Imaging and Analysis of Mitochondrial Dynamics in Living Cells.- Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors.- In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide.- Databases and Informatics Resources For Analysis of Plant Mitochondria.- Expression and Crystallization of the Plant Alternative Oxidase.