Single-Cell Analysis

Methods and Protocols

Häftad, Engelska, 2016

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Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells.

Introduction: Why Analyze Single Cells?.- Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview.- Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry.- Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts.- Single-Cell Culture in Microwells.- Iso-Dielectric Separation and Analysis of Cells.- Single Cell Electroporation Using Microfluidic Devices.- Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array.- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay.- Droplet Microfluidics for Single-Cell Analysis.- Screening of Antigen-Specific Antibody Secreting Cells.- Analysis of Single Eukaryotic Cells Using Raman Tweezers.- Single-Cell Microinjection Technologies.- Ultrasonic Manipulation of Single Cells.- Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices.- Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses.