Cell Senescence

Methods and Protocols

AvLorenzo Galluzzi,Ilio Vitale

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 30 mm
Vikt:1 025 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:538
Förlag:Humana Press Inc.
ISBN:9781493959860

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

Cell Senescence as Both a Dynamic and a Static Phenotype.-Senescence Regulation by mTOR.-Senescence Regulation by the p53 Protein Family.-Markers of Cellular Senescence.-Biomarkers of Cell Senescence Assessed by Imaging Cytometry.-Cytofluorometric Assessment of Cell Cycle Progression.-Quantification of Cell Cycle-arresting Proteins.-Colorimetric Detection of Senescence-associated β Galactosidase (SA-βgal).-Chemiluminescent Detection of Senescence-Associated β Galactosidase (SA-βgal).-Detection of the Senescence-associated Secretory Phenotype (SASP).-Unbiased Characterization of the Senescence-associated Secretome using SILAC-Based Quantitative Proteomics.-Detection of Senescence-associated Heterochromatin Foci (SAHF).-Monitoring DNA Damage During Cell Senescence.-Assessment and Quantification of Telomerase Enzyme Activity.-Methods for the Assessment of Telomere Status.-Detection of Nuclear Envelope Alterations in Senescence.-Measuring Reactive Oxygen Species in Senescent Cells.-Quantification of Protein Carbonylation.-Assays for the Measurement of Lipid Peroxidation.-Raman Spectroscopy for the Detection of AGEs/ALEs.-Monitoring Oncogenic B-RAF-induced Senescence in Melanocytes.-Methods to Investigate the Role of SIRT1 in Endothelial Senescence.-Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1.-Profiling the Metabolic Signature of Senescence.-Genome-wide RNAi Screening to Identify Regulators of Oncogene-induced Cellular Senescence.-An Integrated Approach for Monitoring Cell Senescence.-Robust Multiparametric Assessment of Cellular Senescence.-Assessing Chronological Aging in Bacteria.-Assessing Organismal Aging in the Filamentous Fungus Podospora anserina.-Assessing Chronological Aging in Saccharomyces cerevisiae.-Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis.-High Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditiselegans.-Assessing Senescence in Drosophila using Video Tracking.-Assessing Vascular Senescence in Zebrafish.