Phage Display

Methods and Protocols

AvMichael Hust,Theam Soon Lim

Inbunden, Engelska, 2017

Del i serien Methods in Molecular Biology

2 702 kr

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Beskrivning

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery.

Produktinformation

Utgivningsdatum:2017-11-21
Mått:178 x 254 x 37 mm
Vikt:1 266 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:562
Upplaga:17001
Förlag:Humana Press Inc.
ISBN:9781493974467

Utforska kategorier

Onkologi inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin

Innehållsförteckning

Construction of Human Immune and Naïve scFv Libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of Synthetic Antibody Phage Display Libraries.- Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes.- Construction of Macaque Immune Libraries.- Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format.- Construction of Rabbit Immune Antibody Libraries.- Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries.- Construction of High-Quality Camel Immune Antibody Libraries.- Construction of Chicken Antibody Libraries.- Construction and Selection of Affilin Phage Display Libraries.- Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies.- Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach.- Parallelized Antibody Selection in Microtiter Plates.- Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection.- Phage Display and Selections on Cells.- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.- Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies.- Screening Phage Display Antibody Libraries using Protein Arrays.- Antibody Selection on FFPE Tissue Slides.- Antibody Affinity and Stability Maturation by Error Prone PCR.- Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data.- Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction.- High-Throughput IgG Reformatting and Expressions.- Monitoring Phage Biopanning by Next-Generation Sequencing.- ORFeome Phage Display.- Epitope Mapping by Phage Display.- Metasecretome Phage Display.- Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages.