Epigenome Editing

Methods and Protocols

AvAlbert Jeltsch,Marianne G. Rots

Inbunden, Engelska, 2018

Del i serien Methods in Molecular Biology

1 654 kr

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Beskrivning

This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field.

Produktinformation

Utgivningsdatum:2018-03-10
Mått:178 x 254 x 33 mm
Vikt:1 121 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:482
Upplaga:18001
Förlag:Humana Press Inc.
ISBN:9781493977734

Utforska kategorier

Medicinsk genetik inom Medicin

Innehållsförteckning

Editing the Epigenome: Overview, Open Questions, and Directions of Future Development.- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing.- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities.- Generation of TALE-Based Designer Epigenome Modifiers.- Neuroepigenetic Editing.- Allele-Specific Epigenome Editing.- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox.- CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control.- Delivery of Designer Epigenome Modifiers into Primary Human T Cells.- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques.- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration.- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain.- Non-Viral Methodology for Efficient Co-Transfection.- Chromatin Immunoprecipitation in Human and Yeast Cells.- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis.- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data.- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS).- Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching.- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing.- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions.- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells.- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.