Cell Reprogramming

Methods and Protocols

AvPaul J. Verma,Huseyin Sumer

Häftad, Engelska, 2019

Del i serien Methods in Molecular Biology

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Beskrivning

The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates;

Produktinformation

Utgivningsdatum:2019-03-13
Mått:178 x 254 x 15 mm
Vikt:604 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:271
Förlag:Humana Press Inc.
ISBN:9781493979752

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik

Mer om författaren

Professor Paul J. Verma PhD Principal Scientist- Reproductive Biology South Australian Research Institute (SARDI) Adjunct Professor & Head Cell Reprogramming & Stem Cells Monash Institute of Medical Research 27-31 Wright Street Clayton, VIC 3168 AUSTRALIA Voice: + 61 3 9902 4771 Fax: + 61 3 9594 7416 Cell: +61 413 387 563 Email: Paul.Verma@sa.gov.au Email: Paul.Verma@monash.edu Huseyin Sumer Monash Institute of Medical Research Faculty of Medicine, Nursing and Health Sciences MIMR Centre for Reproduction and Development Phone: 61 2 990 24739 email: huseyin.sumer@monash.edu

Innehållsförteckning

Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine.- Synthetic mRNA Reprogramming of Human Fibroblast Cells.- MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells.- Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors.- Reprogramming of Human Fibroblasts with Non-Integrating RNA Virus on Feeder-Free or Xeno-Free Conditions.- Inducing Pluripotency in Cattle.- Generation of Induced Pluripotent Stem Cells (iPSCs) From Adult Canine Fibroblasts.- Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a PiggyBac Transposon Delivery System and Temporal Control of Transgene Expression.- Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.- Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cells.- Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation.- Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer.- Generation of Chimeras from Porcine Induced Pluripotent Stem Cells.- A Novel Method on Somatic Cell Nuclear Transfer with the Minimum Equipment.- Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer.- Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts.- Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells.- Genome Modification of Pluripotent Cells By Using Transcription Activator-Like Effector Nucleases (TALENs).