Poliovirus

Methods and Protocols

AvJavier Martín

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 202 kr

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Beskrivning

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.

Produktinformation

Utgivningsdatum:2018-04-14
Mått:178 x 254 x 20 mm
Vikt:670 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:341
Förlag:Humana Press Inc.
ISBN:9781493980130

Utforska kategorier

Infektionssjukdomar och smittsamma sjukdomar inom Medicin
Patologi inom Medicin
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication.- Poliovirus Laboratory Based Surveillance: An Overview.- Isolation and Characterisation of Enteroviruses from Clinical Samples.- Isolation and Characterisation of Poliovirus in Cell Culture Systems.- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol.- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays.- A Transgenic Mouse Model of Poliomyelitis.- Standardized Methods for Detection of Poliovirus Antibodies.- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR.- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative.- Phylogenetic Analysis of Poliovirus Sequences.- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA.- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses.- Methods to Monitor Molecular Consistency of Oral Polio Vaccine.- Methods for the Quality Control of Inactivated Poliovirus Vaccines.- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.- Identification and Analysis of Antiviral Compounds against Poliovirus.