This volume containsprotocols specifically designed for studying programmed cell death, and alsodiscusses recent advances in techniques that span broader areas of biology thathave been recently used or that have potential to be incorporated into cell deathresearch. The protocols are mostly described in the context of mammaliansystems, but also cover other systems such as plants, Drosophila, andyeast. Programmed Cell Death: Methods and Protocols is comprised of 20chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9describe methods for studying apoptosis associated with various pathologies indifferent organs including the lymphoid compartment, intestinal epithelium,granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniquesfor studying apoptosis in non-mammalian systems; Chapters 14-16 coverbiochemical and biophysical methods for studying Bcl-2 family protein dynamicsand protein-protein interactions during apoptosis;and the last four chaptersexplore protocols that are useful not only in apoptosis research but in widerareas of biological research, such as genome editing, inducible transgenes, andproteomics. Written in the highly successful Methods in Molecular Biologyaeries format, chapters include introductions to their respective topics, listsof the necessary material and reagents, step-by-step, readily reproduciblelaboratory protocol, and tips on troubleshooting and avoiding known pitfalls.Thoroughand cutting-edge, Programmed Cell Death: Methods and Protocols is acomprehensive and valuable resource for researchers, ranging from beginner toexpert, in their studies on programmed cell death.
