Non-Viral Gene Delivery Vectors

Methods and Protocols

AvGabriele Candiani

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

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Beskrivning

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors.

Produktinformation

Utgivningsdatum:2018-05-30
Mått:178 x 254 x 21 mm
Vikt:608 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:290
Förlag:Humana Press Inc.
ISBN:9781493981182

Utforska kategorier

Anatomi inom Medicin
Medicinsk genetik inom Medicin

Innehållsförteckning

Physical Chemical and BiomolecularMethods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitrofor the Gene Therapy Applications.- Cationic Lipid-Based Nucleic Acid Vectors.- Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPELiposomes and Gemini Micelles.- Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA.- Elaboration and Physicochemical Characterization of Niosome-BasedNioplexes for Gene Delivery Purposes.- Quantitative Intracellular Localization of Cationic Lipid-NucleicAcid Nanoparticles with Fluorescence Microscopy.- TargetedDelivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.- Lipoplexes Strengthened by AnionicPolymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipidsand Anionic Polymers.- Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.- PolyallylamineDerivatives: Novel Non-Toxic Transfection Agents.- BiodegradableThree-Layered Micelles and Injectable Hydrogels.- Cationic Lipid-CoatedPolyplexes (“Lipopolyplexes”) for DNA and Small RNA Delivery.- Preparation of Targeted AnionicLipid-Coated Polyplexes for MicroRNA Delivery.- Characterizationand Investigation of Redox-Sensitive Liposomes for Gene Delivery.- From ArtificialAmino Acids to Sequence-Defined Targeted Oligoaminoamides.- Gene DeliveryMethod Using Photo-Responsive Poly(β-Amino Ester) as Vectors.- Thermo-ResponsivePolyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for SystemicGene Therapy.- Application of Polyethylenimine-Grafted Silicon NanowireArrays for Gene Transfection.