Skeletal Muscle Regeneration in the Mouse

Michael Kyba, Ph.D.Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SEMinneapolis, MN 55455, USA

• EccentricContraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Modelsto Impair Skeletal Muscle’s Capacity to Generate Force.- VolumetricMuscle Loss.- Freeze Injury of the Tibialis AnteriorMuscle.- Synergist Ablationas a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle.- Inducingand Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride.- Cardiotoxin Induced Injury andSkeletal Muscle Regeneration.- Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal SkeletalMuscle.- Isolation, Cryosection, and Immunostainingof Skeletal Muscle.- Isolation of Mouse Periocular Tissuefor Histological and Immunostaining Analyses of the Extraocular Muscles andtheir Satellite Cells.- Skeletal Muscle Tissue Clearingfor LacZ and Fluorescent Reporters, and Immunofluorescence Staining.- Isolation, Culture, FunctionalAssays and Immunofluorescence of Myofiber-Associated Satellite Cells.- Transplantation-Based QuantitativeAssays for Satellite Cell Self-Renewal and Differentiation.- Non-Invasive Tracking of Quiescentand Activated MuSC Engraftment Dynamics In Vivo.- Myogenic Progenitors from Mouse PluripotentStem Cells for Muscle Regeneration.- Assaying Human Myogenic ProgenitorCell Activity by Reconstitution of Muscle Fibers and Satellite Cells in ImmunodeficientMice.- Methods for Mitochondria and MitophagyFlux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle.- Identification, Isolation, andCharacterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle.- FACS Fractionation and Differentiation of Skeletal-MuscleResident Multipotent Tie2+ Progenitors.- In Vitro Assays to DetermineSkeletal Muscle Physiologic Function.- In Vivo Assessment of MuscleContractility in Animal Studies.- Functional Measurement ofRespiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure.- Assessment of the Contractile Propertiesof Permeabilized Skeletal Muscle Fibers.- Analysis of Aerobic Respirationin Intact Skeletal Muscle Tissue by Microplate-Based Respirometry.