Clostridium difficile

Methods and Protocols

AvAdam P. Roberts,Peter Mullany

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

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Beskrivning

This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile.

Produktinformation

Utgivningsdatum:2018-06-09
Mått:178 x 254 x 17 mm
Vikt:576 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:292
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493981762

Utforska kategorier

Onkologi inom Medicin

Innehållsförteckning

Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates.- Direct PCR-ribotyping of Clostridium difficile.- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data.- Clostridium difficile Genome Editing Using pyrE Alleles.- Use of mCherryOpt Fluorescent Protein in Clostridium difficile.- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile.- Clostridium difficile Adhesins.- Intestinal Epithelial Cell Response to Clostridium difficile Flagella.- Isolating and Purifying Clostridium difficile Spores.- Inducing and Quantifying Clostridium difficile Spore Formation.- Characterization of Functional Prophages in Clostridium difficile.- Induction and Purification of C. difficile Phage Tail-like Particles.- Phage Transduction.- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics.- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile.- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains.- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection.- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community.- A Practical Method for Preparation of Fecal Microbiota Transplantation.- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine.- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins.