Clostridium difficile

From the reviews:“This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. … This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. … This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile.” (Rebecca T. Horvat, Doody’s Review Service, October, 2010)

• to Clostridium difficile and the Disease It Causes.- Clostridium difficile: No Longer an Enigmatic Pathogen?.- Clostridium difficile and the Disease It Causes.- Isolation and Culture Techniques.- Clostridium difficile Isolation and Culture Techniques.- Methods for Typing and Epidemiological Studies.- Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping.- Clostridium difficile Toxinotyping.- Multilocus Sequence Typing for Clostridium difficile.- Biochemistry of the Organism.- Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes.- Dissecting the Cell Surface.- Human Intestinal Epithelial Response(s) to Clostridium difficile.- Genomics.- Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays.- Development of Systems for Genetic Analysis of the Organism.- ClosTron-Targeted Mutagenesis.- Methods for Gene Cloning and Targeted Mutagenesis.- Transposon Mutagenesis in Clostridium difficile.- Animal Models of Disease.- Refinement of the Hamster Model of Clostridium difficile Disease.- Methods for Working with the Mouse Model.