Isolation of Plant Organelles and Structures

Methods and Protocols

AvNicolas L. Taylor,A. Harvey Millar

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 233 kr

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Beskrivning

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced.

Produktinformation

Utgivningsdatum:2018-06-27
Mått:178 x 254 x 20 mm
Vikt:663 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:338
Förlag:Humana Press Inc.
ISBN:9781493982288

Utforska kategorier

Växtböcker inom Djur och Natur
Botanik inom Naturvetenskap och teknik

Innehållsförteckning

The Isolation of Plant Organelles and Structures in the Post-Genomics Era.- Approaches to Characterize Organelle, Compartment, or Structure Purity.- Isolation of Nuclei and Nucleoli.- Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts.- Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit.- Leucoplast Isolation and Subfractionation.- Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes.- Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane.- Isolation of Vacuoles and the Tonoplast.- Isolation of Endoplasmic Reticulum and Its Membrane.- Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings.- Isolation of Autolysosomes from Tobacco BY-2 Cells.- Isolation of Protein Storage Vacuoles and Their Membranes.- Isolation of the Cell Wall.- Isolation of Plasmodesmata.- Isolation of Plasma Membrane and Plasma Membrane Microdomains.- Enrichment of the Plant Cytosolic Fraction.- Isolation of Apoplast.- Isolation of Cytosolic Ribosomes.- Isolation of Plastid Ribosomes.- Isolation of Mitochondrial Ribosomes.- Isolation of Microtubules and Microtubule-Associated Proteins.- Isolation of Actin and Actin Binding Proteins.- Purification of 26S Proteasomes and Their Sub-Complexes from Plants.