MicroRNA Detection and Target Identification

Methods and Protocols

AvTamas Dalmay

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 636 kr

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Beskrivning

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization.

Produktinformation

Utgivningsdatum:2018-07-20
Mått:178 x 254 x 18 mm
Vikt:605 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:304
Förlag:Humana Press Inc.
ISBN:9781493983223

Utforska kategorier

Medicinsk genetik inom Medicin

Innehållsförteckning

Improved denaturation of Small RNA Duplexes and its Application for Northern-blotting.- High throughput RT-qPCR for the Analysis of Circulating microRNAs.- Genome-wide Comparison of Next Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum.- Small RNA Profiling by Next Generation Sequencing using High Definition Adapters.- Surface Acoustic Wave Lysis and Lon-exchange Membrane Quantification of Exosomal microRNA.- Droplet Microfluidic Device Fabrication and use for Isothermal Amplification and Detection of microRNA.- Interrogation of Functional miRNA-target Interactions by CRISPR/Cas9 Genome Engineering.- Cell-free Urinary microRNAs Expression in small-scale Experiments.- Peptide-based Isolation of Argonaute Protein Complexes using Ago-APP.- Predicting functional microRNA-mRNA interactions.-Computational and Experimental Identification of Tissue-specific microRNA Targets.- sRNAtoolboxVM: Small RNA Analysis in a Virtual Machine.- An Assessment of the Next Generation of Animal miRNA Target Prediction Algorithms.- The UEA Small RNA Workbench: A Suite of Computational Tools for Small RNA Analysis.- Prediction of miRNA-mRNA Interactions using miRGate.- Detection of microRNAs using Chip-based QuantStudio 3D Digital PCR.- MiRNA Quantitation with Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification.- A Robust Protocol to Quantify Circulating Cancer Biomarker microRNAs.- MicroRNAs, Regulatory Networks and Comorbidities: Decoding Complex Systems.- Label-free Direct Detection of MiRNAs with Poly-silicon Nanowire Biosensors.