Cancer Stem Cells

Methods and Protocols

AvGianpaolo Papaccio,Vincenzo Desiderio

Häftad, Engelska, 2018

Del i serien Methods in Molecular Biology

1 530 kr

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Beskrivning

This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells.

Produktinformation

Utgivningsdatum:2018-05-17
Mått:178 x 254 x 14 mm
Vikt:460 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:223
Förlag:Humana Press Inc.
ISBN:9781493984749

Utforska kategorier

Onkologi inom Medicin
Rättsmedicin inom Medicin

Innehållsförteckning

Introduction to Cancer Stem Cells: Past, Present, and Future.- Surface Markers for the Identification of Cancer Stem Cells.- The Role of CD44 and Cancer Stem Cells.- Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay.- Isolation of Cancer Stem Cells by Side Population Method.- Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres.- In Vitro Tumorigenic Assay: The Tumor Spheres Assay.- In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells.- Xenograft as In Vivo Experimental Model.- How to Assess Drug Resistance in Cancer Stem Cells.- Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells.- Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML).- Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System.- Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform.- Assessing DNA Methylation in Cancer Stem Cells.- Histones Acetylation and Cancer Stem Cells (CSCs).- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods.- Circulating Tumor Cells.