The methods described in the second edition on
Legionella are for the study of distinct features of
L. pneumophila. Chapters guide readers through ecology and physiology of
legionella, legionella genetics, cellular microbiology of
legionella, biochemical assays to study
legionella effectors and enzymes, immunity and host response against
legionella, metagenomics, proteomics, and host microbiomes of
legionella. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.