Electroporation Protocols

Microorganism, Mammalian System, and Nanodevice

AvShulin Li,Lingqian Chang

Häftad, Engelska, 2020

Del i serien Methods in Molecular Biology

2 380 kr

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Beskrivning

This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease.

Produktinformation

Utgivningsdatum:2020-08-30
Mått:178 x 254 x 12 mm
Vikt:402 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:196
Upplaga:3
Förlag:Humana Press Inc.
ISBN:9781493997428

Utforska kategorier

Medicinsk genetik inom Medicin

Innehållsförteckning

Cell Size-specific Transfection by Micropillar Array Electroporation.- Soft Electroporation through 3D Hollow Nanoelectrodes.- Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device.- 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.- Low-Voltage Flow-Through Electroporation Membrane and Method.- Nanofountain Probe Electroporation for Monoclonal Cell Line Generation.- 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery.- Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells.- Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout.- Microfluidic Device for Localized Electroporation.- Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation.- Targeted in vivo Electroporation Using Nanoengineered Microelectrodes.- Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice.- Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection.- Tissue Nano-Transfection of Surgically Exposed Stroma.- Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal.- Electroporation Transformation Protocol for Phaeodactylum tricornutum.- Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation.- Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica.- Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp..- Electrotransformation of Saccharomyces cerevisiae.