Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USAMarkus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany

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• LABORATORY PROCEDURES 1 The documentation systemMarkus Montag and Lars Johansson2 Culture Media TechniquesLars Johansson3 IVF incubator handlingJason E Swain and Alex Lagunov4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC)Markus Montag5 Semen collection and sample receptionCarin Huyser6 Spermiogramm, Makler chamber, Neubauer chamber, CASAVerena Nordhoff7 Sperm viability testingMarkus Montag8 Sperm preparation – Swim up, density gradient, migration chamber, zeta methodThomas Ebner9 Sperm selection using hyaluronan bindingDenny Sakkas10 IVF - InseminationMarkus Montag11 ICSIThomas Freour12 Oocyte in vitro maturation – Isolation of COCs and culture for maturationMariabeatrice Dal Canto and Giovanni Coticchio13 Artificial oocyte activationMarkus Montag14 Cryopreservation - Sperm TESE and MESABorut Kovačič15 Vitrification – Oocytes, 2PN, Embryos, BlastocystsAna Cobo and Aila Coello16 Slow freezing: principles, oocytes, 2PN, embryos, blastocystsEtienne Van den Abbeel17 Oocyte selectionBaşak Balaban18 Zygote selectionMartin Greuner19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6Thorir Hardarson and Julius Hreinsson20 Time-lapse imagingCristina Hickman21 Assisted hatchingMaria De Geyter and Christian De Geyter22 Zona manipulation at various embryo stages: Mechanical / acidic mediumMarius Meintjes23 Biopsy: polar body, blastomere, trophectodermHakan Kadir Yelke and Semra Kahraman24 Sampling of cells after biopsy for aCGHBarry Behr25 Embryo transfer – standard and ultrasound guidedBettina Toth26 Hygenic standards in the IVF laboratoryVera Baukloh27 Quality control of IVF laboratory supplies – Mouse Embryo Assay (MEA)Dean E Morbeck28 Troubleshooting in the IVF laboratoryDavid Mortimer and Sharon T Mortimer29 MicroscopyMarkus Montag30 Fertility preservationMarkus Montag and Jana LiebenthronCLINICAL PROCEDURES31 Ultrasound evaluation in treatment managementIppokratis Sarris and Geeta Nargund 32 Three-Dimensional Ultrasound and the Diagnosis of Uterine AnomaliesMagued Adel Aziz Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk 33 Infertility ultrasound evaluation: One Stop Procedure Spiros A. Liatsikos and Geeta Nargund34 Office Hysteroscopy Linda D. Bradley35 Hysteroscopic management of uterine septumOmar Abuzeid and Mostafa Abuzeid 36 Hysteroscopic Lysis of Intrauterine AdhesionsBolonduro Oluwamuyiwa and Mostafa Abuzeid 37 Hysteroscopic Management of Submucosal FibroidsSalem Joseph and Mostafa Abuzeid38 Saline infusion sonohysterographyAlan Bolnick and Mostafa Abuzeid39 General Principles of LaparoscopyRubin Raju and Mostafa Abuzeid40 Microlaparoscopy Oscar D. Almeida Jr41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy Mostafa Abuzeid and Ahmed Abdel Aziz42 Laparoscopic Excision of a Large EndometriomaRubin Raju and Mostafa Abuzeid43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosisOmar Abuzeid and Mostafa Abuzeid 44 Laparoscopic management of mature cystic teratomaRahima Sanya and Mostafa Abuzeid 45 Tubal Cannulation for Proximal Tubal DiseaseBotros Rizk and Josh Ekladios 46 Robotic assisted laparoscopic myomectomy Hashem Lofty, Arsany Bassily, Lauren Mann, and Botros Rizk 47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization Hashem Lofty, Rola Turki, and Botros Rizk48 Unexplained infertility Hassan N. Sallam and Nooman H. Sallam 49 Male Evaluation for InfertilityKiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal50 HysterosalpingographyIslam Fahmi, Mostafa Abuzeid, and Shawky ZA Badawy 51 Intrauterine insemination: clinical perspectives Willem Ombelet 52 GnRH agonist to trigger final follicular maturation in IVFNayana Talukdar and Robert F. Casper53 GnRH agonist Protocols Justin Tan, Rabea Youcef Khoudja, Michael Doley, Michael Dahan and Seang Lin Tan 54 GnRH agonists before IVF for advanced endometriosisHassan N. Sallam and Nooman H. Sallam55 Microdose GnRH Agonist Flare ProtocolsMagued Adel Mikhail, Wael Salem, and Botros Rizk 56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization Michael Dahan, Justin Tan, Baris Ata, Rabea Khoudja, Seang Lin Tan, and Botros Rizk57 Minimal stimulation for IVF Ippokratis Sarris and Geeta Nargund 58 Ovarian Hyperstimulation SyndromeMagued Adel Mikhail, Daniel Antonious, Candice P. Holliday, and Botros Rizk59 The Prediction of OHSS Jan Gerris and Jana Claeys 60 Tips on technique of embryo transferOmar Abuzeid and Mostafa Abuzeid 61 Difficult Embryo Transfers Gautam N. Allahbadia and Goral N. Gandhi62 In vitro maturation of oocytes for fertility treatment and fertility preservationMichael H. Dahan, Justin Tan, Rabea Youcef Khoudja, Andrew Mok, and Seang Lin Tan63 Complications and outcomes of IVFJan Gerris64 Obstetric complications after in-vitro fertilizationBrian Brocato and David Lewis 65 Twins pregnancy after ARTDavid F. Lewis and Brian Brocato 66 IVF and endometriosisGraciela Kohls Ilgner and Juan Antonio Garcia Velasco 67 Leiomyoma of the Uterus Shawky ZA Badawy68 Office Management of Polycystic Ovarian SyndromeShawky ZA Badawy 69 Adenomyosis: Imaging and TreatmentMagued Adel Mikhail, Candice P. Holliday, and Botros Rizk70 Ectopic Pregnancy: Evaluation and Management James P. Schwayder