Molecular Diagnosis of Cancer

"...concise and uniformly well written...contains a large compilation of applications of nucleic acid technologies that are in use or may be in use in the near future to diagnose and aid in the management of malignant diseases." - Doody's Health Sciences Book Review Journal Review of the First Edition The techniques described here are standard laboratory lore;the added value are the notes and tips on tricky details andoften overlooked precautions...Laboratory scientists...will get their money's worth. -Modern Pathology "...a diverse collection of readily reproducible methods...the protocols presented follow step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls." - Tumori From the reviews of the second edition: "Dramatic advances in molecular biology over the past decades have led to an unprecedented understanding of cancer at a very detailed molecular level. ... tips on troubleshooting are presented in each chapter, written by experts in the field. Given the amount of detail incorporated in each protocol, these should be readily reproducible and enable laboratories with no prior experience in a specific technique to master it and be able to use todays most powerful techniques in the molecular diagnosis of cancer." (Janis Racevskis, Medical Oncology, Vol. 22 (3), 2004)

• Prognostic and Predictive Factors.- Assessment of Predictive Values of Tumor Markers.- Quality Assurance of Predictive Markers in Breast Cancer.- Extraction of Nucleic Acid Templates.- Microdissection and Extraction of DNA From Archival Tissue.- Fluorescence In Situ Hybridization.- HER2 FISH in Breast Cancer.- Fluorescence In Situ Hybridization for BCR-ABL.- UroVysion™ Multiprobe FISH in Urinary Cytology.- Chromogenic In Situ Hybridization in Tumor Pathology.- Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia.- Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization.- A Nested RT-PCR Assay to Detect BCR/abl.- TP53 Mutation Detection by SSCP and Sequencing.- PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies.- Circulating DNA Analysis.- Microsatellite Instability.- Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disorders.- Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia.- Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reaction.- Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR.- Detection of Telomerase Enzyme Activity by TRAP Assay.- Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarrays.- Detection of K-ras Mutations by a Microelectronic DNA Chip.- Microarray-Based CGH in Cancer.- Tissue Microarrays.