Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. The protocols eliminate much of the chore of adapting techniques initially developed for animal cell culture systems or the time spent in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. They allow researchers to use successfully high quality in vitro cultures as models to explore the disease process.