Microtubule Protocols

Häftad, Engelska, 2010

Del i serien Methods in Molecular Medicine

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Beskrivning

Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes.

Produktinformation

Utgivningsdatum:2010-11-10
Mått:152 x 229 x 18 mm
Vikt:463 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Medicine
Antal sidor:305
Förlag:Humana Press Inc.
ISBN:9781617376917

Utforska kategorier

Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik
Kemi inom Naturvetenskap och teknik

Innehållsförteckning

Microtubules.- Large Scale Purification of Brain Tubulin With the Modified Weisenberg Procedure.- Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts.- Preparation and Characterization of Posttranslationally Modified Tubulins From Artemia franciscana.- Studying the Structure of Microtubules by Electron Microscopy.- Fluorescence Microscopy of Microtubules in Cultured Cells.- Measurements of Stathmin-Tubulin Interaction in Solution.- Analysis of Microtubule Dynamics by Polarized Light.- Visualization of Spindle Behavior Using Confocal Microscopy.- Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila.- Analysis of Tubulin Transport in Nerve Processes.- Analysis of Microtubule-Mediated Intracellular Viral Transport.- Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions.- Screening for Inhibitors of Microtubule-Associated Motor Proteins.- Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy.- Studying Drug-Tubulin Interactions by X-Ray Crystallography.- Characterizing Ligand-Microtubule Binding by Competition Methods.- Methods for Studying Vinca Alkaloid Interactions With Tubulin.- High-Throughput Screening of Microtubule-Interacting Drugs.- Strategies for the Development of Novel Taxol-Like Agents.