This book constitutes the revised selected papers of the 44th International Workshop on Graph-Theoretic Concepts in Computer Science, WG 2018, held  in Cottbus, Germany, in June 2018.The 30 full papers presented in this volume were carefully reviewed and selected from 66 submissions. They cover a wide range of areas, aiming at connecting theory and applications by demonstrating how graph-theoretic concepts can be applied in various areas of computer science. Another focus is on presenting recent results and on identifying and exploring promising directions of future research.