This book constitutes the thoroughly refereed proceedings of the Second International Workshop of Computational Neuroscience, held in São João Del-Rei, Brazil, in September 2019.The 17 full papers and 3 short papers presented have been thoroughly reviewed and selected from 45 submissions. The papers are organized in topical sections: artificial intelligence, machine learning, and related topics; complex systems and complex networks; computational neuroscience of learning and memory; neural signal processing; software and hardware implementations in neuroscience; brain-machine interfaces and neurostimulation; and seizure prediction.