This two-set volume LNAI 16187 and 16188 constitutes the refereed proceedings of the 27th International Conference on Speech and Computer SPECOM 2025 held in Szeged, Hungary, during October 13–15, 2025.The 47 full papers and 1 invited paper included in this book were carefully reviewed and selected from 77 submissions. The papers are organized in the following topical sections: Part I- Invited Paper; Speech Perception and Synthesis; Computational Paralinguistics; Speech Processing for Healthcare; Speech and Language Resources; Speaker Recognition.Part II- Automatic Speech Recognition; Speech Processing for Under-Resourced Languages; Digital Speech Processing; Natural Language Processing; Multimodal Systems.