This book constitutes the refereed post-conference proceedings of the 16th International Conference on Artificial Evolution, EA 2024, held in Bordeaux, France, during October 29–31, 2024.The 16 full papers were carefully reviewed and selected from 30 submissions. The papers cover a wide range of topics in the field of artificial evolution, including Algorithmics and Modeling, Implementations, Application of Evolutionary Paradigms to the Real World industry, biosciences, Machine Learning and hybridization with other soft computing techniques.