Markus Sauer studied chemistry in Karlsruhe, Saarbrucken, and Heidelberg, and finished his PhD in Physical Chemistry at the University of Heidelberg under the guidance of Prof. Jurgen Wolfrum. After a short-term visit at LBNL, Berkeley in the group of Prof. Shimon Weiss, he was decorated with the BioFuture award to perform independent research on single-molecule handling, detection, and identification. He finished his habilitation in Heidelberg at the Institute of Physical Chemistry and is now Professor for Applied Laser Physics and Laser Spectroscopy at the University in Bielefeld. Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) in Chemistry from the University of Leuven (K.U.Leuven). After postdoctoral research with Prof. Masuhara at Osaka University and Prof. Barbara at the University of Minneapolis, he rejoined the K.U.Leuven supervising the Single Molecule Unit in the group of Prof. De Schryver. In 2005 he was appointed Research Professor at the K.U.Leuven and recently he was promoted to full professor. His research interests are fast spectroscopy, (single molecule) fluorescence microscopy and nanoscopy and the application of these techniques in material science and biosciences.

"Therefore, this book represents a valuable source of information for both specialists in single moleculefluorescence spectroscopy and researchers new to this branch of fluorescence techniques." (Anal Bioanal Chem, 12 February 2012)

• Basic Principles of fluorescence spectroscopyFluorophores and Fluorescent LabelsFluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS)Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching PathwaysFluorescence Correlation SpectroscopyExcited state energy transfer Photoinduced Electron Transfer (PET) ReactionsSuper-Resolution Fluorescence ImagingSingle-molecule enzymatics

Markus Sauer studied chemistry in Karlsruhe, Saarbrucken, and Heidelberg, and finished his PhD in Physical Chemistry at the University of Heidelberg under the guidance of Prof. Jurgen Wolfrum. After a short-term visit at LBNL, Berkeley in the group of Prof. Shimon Weiss, he was decorated with the BioFuture award to perform independent research on single-molecule handling, detection, and identification. He finished his habilitation in Heidelberg at the Institute of Physical Chemistry and is now Professor for Applied Laser Physics and Laser Spectroscopy at the University in Bielefeld. Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) in Chemistry from the University of Leuven (K.U.Leuven). After postdoctoral research with Prof. Masuhara at Osaka University and Prof. Barbara at the University of Minneapolis, he rejoined the K.U.Leuven supervising the Single Molecule Unit in the group of Prof. De Schryver. In 2005 he was appointed Research Professor at the K.U.Leuven and recently he was promoted to full professor. His research interests are fast spectroscopy, (single molecule) fluorescence microscopy and nanoscopy and the application of these techniques in material science and biosciences.

"Therefore, this book represents a valuable source of information for both specialists in single moleculefluorescence spectroscopy and researchers new to this branch of fluorescence techniques." (Anal Bioanal Chem, 12 February 2012)

• Basic Principles of fluorescence spectroscopyFluorophores and Fluorescent LabelsFluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS)Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching PathwaysFluorescence Correlation SpectroscopyExcited state energy transfer Photoinduced Electron Transfer (PET) ReactionsSuper-Resolution Fluorescence ImagingSingle-molecule enzymatics

Markus Sauer studied chemistry in Karlsruhe, Saarbrucken, and Heidelberg, and finished his PhD in Physical Chemistry at the University of Heidelberg under the guidance of Prof. Jurgen Wolfrum. After a short-term visit at LBNL, Berkeley in the group of Prof. Shimon Weiss, he was decorated with the BioFuture award to perform independent research on single-molecule handling, detection, and identification. He finished his habilitation in Heidelberg at the Institute of Physical Chemistry and is now Professor for Applied Laser Physics and Laser Spectroscopy at the University in Bielefeld. Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) in Chemistry from the University of Leuven (K.U.Leuven). After postdoctoral research with Prof. Masuhara at Osaka University and Prof. Barbara at the University of Minneapolis, he rejoined the K.U.Leuven supervising the Single Molecule Unit in the group of Prof. De Schryver. In 2005 he was appointed Research Professor at the K.U.Leuven and recently he was promoted to full professor. His research interests are fast spectroscopy, (single molecule) fluorescence microscopy and nanoscopy and the application of these techniques in material science and biosciences.

"Therefore, this book represents a valuable source of information for both specialists in single moleculefluorescence spectroscopy and researchers new to this branch of fluorescence techniques." (Anal Bioanal Chem, 12 February 2012)

• Basic Principles of fluorescence spectroscopyFluorophores and Fluorescent LabelsFluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS)Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching PathwaysFluorescence Correlation SpectroscopyExcited state energy transfer Photoinduced Electron Transfer (PET) ReactionsSuper-Resolution Fluorescence ImagingSingle-molecule enzymatics